Significance of peripheral mononuclear cells producing interferon-γ in response to insulin B:9–23-related peptides in subtypes of type 1 diabetes

Yoichi Oikawa, Kumiko Sakamoto, Atsushi Satomura, Akifumi Haisa, Takeshi Katsuki, Yutaka Hattori, Ikuo Inoue, Mitsuhiko Noda, Akira Shimada

Research output: Contribution to journalArticle

Abstract

Type 1 diabetes is largely caused by β-cell destruction through anti-islet autoimmunity. Reportedly, interferon (IFN)-γ-secreting peripheral blood mononuclear cells (PBMCs) specific to four insulin B-chain amino acid 9–23-related peptides (B:9–23rPep) were increased in type 1 diabetes participants. This study aimed to investigate the PBMC frequencies in subtypes of type 1 diabetes using enzyme-linked immunospot assay. In this cross-sectional study, peripheral blood samples were obtained from 148 participants including 72 with acute-onset type 1 diabetes (AT1D), 51 with slowly progressive insulin-dependent diabetes mellitus (SPIDDM), and 25 with type 2 diabetes. The frequency of B:9–23rPep-specific IFN-γ-producing PBMCs was significantly higher in AT1D participants than in SPIDDM and type 2 diabetes participants. Meanwhile, a significant inverse correlation was observed between the PMBC frequencies and insulin secretion capacity in SPIDDM participants. These findings suggest that the increased peripheral B:9–23rPep-specific IFN-γ immunoreactivity reflects decreased functional β-cell mass and greater disease activity of type 1 diabetes.

Original languageEnglish
Article number108260
JournalClinical Immunology
Volume208
DOIs
Publication statusPublished - 2019 Nov

Fingerprint

Type 1 Diabetes Mellitus
Interferons
Peptides
Blood Cells
Amino Acids
Type 2 Diabetes Mellitus
Insulin
Enzyme-Linked Immunospot Assay
B-insulin
Autoimmunity
Cross-Sectional Studies

Keywords

  • Autoimmune
  • Enzyme-linked immunospot assay
  • Insulin
  • Interferon-γ
  • Type 1 diabetes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Significance of peripheral mononuclear cells producing interferon-γ in response to insulin B:9–23-related peptides in subtypes of type 1 diabetes. / Oikawa, Yoichi; Sakamoto, Kumiko; Satomura, Atsushi; Haisa, Akifumi; Katsuki, Takeshi; Hattori, Yutaka; Inoue, Ikuo; Noda, Mitsuhiko; Shimada, Akira.

In: Clinical Immunology, Vol. 208, 108260, 11.2019.

Research output: Contribution to journalArticle

Oikawa, Yoichi ; Sakamoto, Kumiko ; Satomura, Atsushi ; Haisa, Akifumi ; Katsuki, Takeshi ; Hattori, Yutaka ; Inoue, Ikuo ; Noda, Mitsuhiko ; Shimada, Akira. / Significance of peripheral mononuclear cells producing interferon-γ in response to insulin B:9–23-related peptides in subtypes of type 1 diabetes. In: Clinical Immunology. 2019 ; Vol. 208.
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