TY - JOUR
T1 - Significance of PGP9.5 expression in cancer-associated fibroblasts for prognosis of colorectal carcinoma
AU - Akishima-Fukasawa, Yuri
AU - Ino, Yoshinori
AU - Nakanishi, Yukihiro
AU - Miura, Ayaka
AU - Moriya, Yoshihiro
AU - Kondo, Tadashi
AU - Kanai, Yae
AU - Hirohashi, Setsuo
PY - 2010/7
Y1 - 2010/7
N2 - To assess the expression of a cancer-associated fibroblasts (CAFs) marker as an indicator of prognosis, we raised anti-protein gene product 9.5 (PGP9.5) monoclonal antibody against cultured fibroblasts. PGP9.5 expression in cultured normal fibroblasts was increased by transforming growth factor β stimulation, indicating the phenotypic alteration to activated fibroblast. We immunohistochemically evaluated PGP9.5 expression with the CAFs of 110 colorectal cancer cases under T3 stage. PGP9.5 immunoreactivity in 30% or more of CAFs was defined as high PGP9.5 expression, and the other cases were considered as having low PGP9.5 expression. Patients with high PGP9.5 expression (42.7%) had significantly shorter survival and a higher incidence of recurrence than the low PGP9.5 expression group (P = .002 and P < .001, respectively). Multivariate analysis indicated PGP9.5 expression as an independent prognostic factor for overall and recurrence-free survival partly as well as lymph node metastasis. These results indicate that PGP9.5 expression in CAFs is a helpful finding to represent the overall biologic behavior of advanced colorectal cancer.
AB - To assess the expression of a cancer-associated fibroblasts (CAFs) marker as an indicator of prognosis, we raised anti-protein gene product 9.5 (PGP9.5) monoclonal antibody against cultured fibroblasts. PGP9.5 expression in cultured normal fibroblasts was increased by transforming growth factor β stimulation, indicating the phenotypic alteration to activated fibroblast. We immunohistochemically evaluated PGP9.5 expression with the CAFs of 110 colorectal cancer cases under T3 stage. PGP9.5 immunoreactivity in 30% or more of CAFs was defined as high PGP9.5 expression, and the other cases were considered as having low PGP9.5 expression. Patients with high PGP9.5 expression (42.7%) had significantly shorter survival and a higher incidence of recurrence than the low PGP9.5 expression group (P = .002 and P < .001, respectively). Multivariate analysis indicated PGP9.5 expression as an independent prognostic factor for overall and recurrence-free survival partly as well as lymph node metastasis. These results indicate that PGP9.5 expression in CAFs is a helpful finding to represent the overall biologic behavior of advanced colorectal cancer.
KW - Cancer-associated fibroblast
KW - Colorectal cancer
KW - Immunohistochemistry
KW - PGP9.5
KW - Prognosis
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UR - http://www.scopus.com/inward/citedby.url?scp=77954466410&partnerID=8YFLogxK
U2 - 10.1309/AJCPRJP39MIDSGBH
DO - 10.1309/AJCPRJP39MIDSGBH
M3 - Article
C2 - 20551269
AN - SCOPUS:77954466410
VL - 134
SP - 71
EP - 79
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
SN - 0002-9173
IS - 1
ER -
