Simian virus 40 large T antigen targets the microtubule-stabilizing protein TACC2

Shuchin Tei, Noriko Saitoh, Tetsushi Funahara, Shin Ichi Iida, Yuko Nakatsu, Kayo Kinoshita, Yoshikazu Kinoshita, Hideyuki Saya, Mitsuyoshi Nakao

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The large T antigens of polyomaviruses target cellular proteins that control fundamental processes, including p53 and the RB family of tumor suppressors. Mechanisms that underlie Tantigen- induced cell transformation need to be fully addressed, because as-yet unidentified target proteins might be involved in the process. In addition, recently identified polyomaviruses are associated with particular human diseases such as aggressive skin cancers. Here, we report that simian virus 40 (SV40) large T antigen interacts with the transforming acidic coiled-coilcontaining protein TACC2, which is involved in stabilizing microtubules in mitosis. T antigen directly binds TACC2 and induces microtubule dysfunction, leading to disorganized mitotic spindles, slow progression of mitosis and chromosome missegregation. These mitotic defects are caused by N-terminaldeleted T antigen, which minimally interacts with TACC2, whereas T-antigen-induced microtubule destabilization is suppressed by overexpressing TACC2. Thus, TACC2 might be a key target of T antigen to disrupt microtubule regulation and chromosomal inheritance in the initiation of cell transformation.

Original languageEnglish
Pages (from-to)3190-3198
Number of pages9
JournalJournal of Cell Science
Volume122
Issue number17
DOIs
Publication statusPublished - 2009 Sep 1

Keywords

  • Chromosomes
  • Large T antigen
  • Mitosis
  • Nucleus
  • TACC2

ASJC Scopus subject areas

  • Cell Biology

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    Tei, S., Saitoh, N., Funahara, T., Iida, S. I., Nakatsu, Y., Kinoshita, K., Kinoshita, Y., Saya, H., & Nakao, M. (2009). Simian virus 40 large T antigen targets the microtubule-stabilizing protein TACC2. Journal of Cell Science, 122(17), 3190-3198. https://doi.org/10.1242/jcs.049627