Simple and rapid determination of serum carbapenem concentrations by high-performance liquid chromatography

Yuji Kurihara, Junko Kizu, Seiji Hori

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Although several high-performance liquid chromatography (HPLC) methods for the determination of serum concentrations of carbapenems have been reported, they are complicated and involve column switching. We established a simple and rapid HPLC method for the determination of serum concentrations of carbapenems, and this method is suitable for routine use in the clinical field. With our HPLC method, the serum concentrations of five commercially available carbapenems could be determined by changing only the methanol/phosphate-buffer ratio in the mobile phase. Serum levels of imipenem, panipenem, and meropenem in mice could be monitored when these carbapenems (20 mg/kg) were administered subcutaneously with cilastatin (20 mg/kg). These results suggest that our simple and rapid HPLC method for the determination of the serum concentrations of carbapenems is useful for pharmacokinetic/pharmacodynamic (PK/PD)-based determination of carbapenem dosage regimens.

Original languageEnglish
Pages (from-to)30-34
Number of pages5
JournalJournal of Infection and Chemotherapy
Volume14
Issue number1
DOIs
Publication statusPublished - 2008 Feb

Keywords

  • Carbapenems
  • High-performance liquid chromatography
  • Pharmacokinetics

ASJC Scopus subject areas

  • Microbiology (medical)
  • Pharmacology (medical)
  • Infectious Diseases

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