Simple and Rapid Staining for Detection of Entamoeba Cysts and Other Protozoans with Fluorochromes

Fumihiko Kawamoto, Sahoko Mizuno, Hisashi Fujioka, Nobuo Kumada, Etsuro Sugiyama, Tsutomu Takeuchi, Seiki Kobayashi, Motohiro Iseki, Minoru Yamada, Yoshitsugu Matsumoto, Tatsuya Tegoshi, Yukio Yoshida

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Three fluorochromes were applied to stain various parasitic protozoans. By double staining with 4′,6-diamidino-2-phenylindole and propidium iodide, differentiation of the nuclei from the cytoplasm can easily be achieved within several seconds. The chromatoid bodies in Entamoeba cysts were stained bright red. Plasmodium yoelii at all stages except late trophozoites and young gametocytes was easily identified. In the oocysts of Cryptosporidium sp., the nuclei and cytoplasm of the sporozoites fluoresced bluish white and red, respectively, whereas the residual body appeared blue or green. The third fluorochrome, Calcofluor white M2R, was suitable for detecting the cysts of Entamoeba spp. and Chilomastix mesnili.

Original languageEnglish
Pages (from-to)35-46
Number of pages12
JournalJapanese Journal of Medical Science and Biology
Volume40
Issue number1
DOIs
Publication statusPublished - 1987

Fingerprint

Entamoeba
Fluorescent Dyes
Cysts
Retortamonadidae
Cytoplasm
Plasmodium yoelii
Staining and Labeling
Sporozoites
Trophozoites
Cryptosporidium
Oocysts
Propidium
Coloring Agents
2-phenylindole
Green Or
C.I. Fluorescent Brightening Agent 28

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Simple and Rapid Staining for Detection of Entamoeba Cysts and Other Protozoans with Fluorochromes. / Kawamoto, Fumihiko; Mizuno, Sahoko; Fujioka, Hisashi; Kumada, Nobuo; Sugiyama, Etsuro; Takeuchi, Tsutomu; Kobayashi, Seiki; Iseki, Motohiro; Yamada, Minoru; Matsumoto, Yoshitsugu; Tegoshi, Tatsuya; Yoshida, Yukio.

In: Japanese Journal of Medical Science and Biology, Vol. 40, No. 1, 1987, p. 35-46.

Research output: Contribution to journalArticle

Kawamoto, F, Mizuno, S, Fujioka, H, Kumada, N, Sugiyama, E, Takeuchi, T, Kobayashi, S, Iseki, M, Yamada, M, Matsumoto, Y, Tegoshi, T & Yoshida, Y 1987, 'Simple and Rapid Staining for Detection of Entamoeba Cysts and Other Protozoans with Fluorochromes', Japanese Journal of Medical Science and Biology, vol. 40, no. 1, pp. 35-46. https://doi.org/10.7883/yoken1952.40.35
Kawamoto, Fumihiko ; Mizuno, Sahoko ; Fujioka, Hisashi ; Kumada, Nobuo ; Sugiyama, Etsuro ; Takeuchi, Tsutomu ; Kobayashi, Seiki ; Iseki, Motohiro ; Yamada, Minoru ; Matsumoto, Yoshitsugu ; Tegoshi, Tatsuya ; Yoshida, Yukio. / Simple and Rapid Staining for Detection of Entamoeba Cysts and Other Protozoans with Fluorochromes. In: Japanese Journal of Medical Science and Biology. 1987 ; Vol. 40, No. 1. pp. 35-46.
@article{4ee7b6cfc4a84c40bcf3f79d9b6f4c3d,
title = "Simple and Rapid Staining for Detection of Entamoeba Cysts and Other Protozoans with Fluorochromes",
abstract = "Three fluorochromes were applied to stain various parasitic protozoans. By double staining with 4′,6-diamidino-2-phenylindole and propidium iodide, differentiation of the nuclei from the cytoplasm can easily be achieved within several seconds. The chromatoid bodies in Entamoeba cysts were stained bright red. Plasmodium yoelii at all stages except late trophozoites and young gametocytes was easily identified. In the oocysts of Cryptosporidium sp., the nuclei and cytoplasm of the sporozoites fluoresced bluish white and red, respectively, whereas the residual body appeared blue or green. The third fluorochrome, Calcofluor white M2R, was suitable for detecting the cysts of Entamoeba spp. and Chilomastix mesnili.",
author = "Fumihiko Kawamoto and Sahoko Mizuno and Hisashi Fujioka and Nobuo Kumada and Etsuro Sugiyama and Tsutomu Takeuchi and Seiki Kobayashi and Motohiro Iseki and Minoru Yamada and Yoshitsugu Matsumoto and Tatsuya Tegoshi and Yukio Yoshida",
year = "1987",
doi = "10.7883/yoken1952.40.35",
language = "English",
volume = "40",
pages = "35--46",
journal = "Japanese Journal of Infectious Diseases",
issn = "1344-6304",
publisher = "National Institute of Health",
number = "1",

}

TY - JOUR

T1 - Simple and Rapid Staining for Detection of Entamoeba Cysts and Other Protozoans with Fluorochromes

AU - Kawamoto, Fumihiko

AU - Mizuno, Sahoko

AU - Fujioka, Hisashi

AU - Kumada, Nobuo

AU - Sugiyama, Etsuro

AU - Takeuchi, Tsutomu

AU - Kobayashi, Seiki

AU - Iseki, Motohiro

AU - Yamada, Minoru

AU - Matsumoto, Yoshitsugu

AU - Tegoshi, Tatsuya

AU - Yoshida, Yukio

PY - 1987

Y1 - 1987

N2 - Three fluorochromes were applied to stain various parasitic protozoans. By double staining with 4′,6-diamidino-2-phenylindole and propidium iodide, differentiation of the nuclei from the cytoplasm can easily be achieved within several seconds. The chromatoid bodies in Entamoeba cysts were stained bright red. Plasmodium yoelii at all stages except late trophozoites and young gametocytes was easily identified. In the oocysts of Cryptosporidium sp., the nuclei and cytoplasm of the sporozoites fluoresced bluish white and red, respectively, whereas the residual body appeared blue or green. The third fluorochrome, Calcofluor white M2R, was suitable for detecting the cysts of Entamoeba spp. and Chilomastix mesnili.

AB - Three fluorochromes were applied to stain various parasitic protozoans. By double staining with 4′,6-diamidino-2-phenylindole and propidium iodide, differentiation of the nuclei from the cytoplasm can easily be achieved within several seconds. The chromatoid bodies in Entamoeba cysts were stained bright red. Plasmodium yoelii at all stages except late trophozoites and young gametocytes was easily identified. In the oocysts of Cryptosporidium sp., the nuclei and cytoplasm of the sporozoites fluoresced bluish white and red, respectively, whereas the residual body appeared blue or green. The third fluorochrome, Calcofluor white M2R, was suitable for detecting the cysts of Entamoeba spp. and Chilomastix mesnili.

UR - http://www.scopus.com/inward/record.url?scp=0023286105&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023286105&partnerID=8YFLogxK

U2 - 10.7883/yoken1952.40.35

DO - 10.7883/yoken1952.40.35

M3 - Article

C2 - 3626134

AN - SCOPUS:0023286105

VL - 40

SP - 35

EP - 46

JO - Japanese Journal of Infectious Diseases

JF - Japanese Journal of Infectious Diseases

SN - 1344-6304

IS - 1

ER -