Simple and robust screening of pooled yeast artificial chromosome libraries by the restriction enzyme digestion of polymerase chain reaction products

Xueqian Wang, Jing Qian, Minoru Ko

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The polymerase chain reaction (PCR)-based method for screening pooled yeast artificial chromosome (YAC) libraries was modified by adding a step for the restriction enzyme digestion of the PCR products. This modification significantly increased the reliability of YAC screening and made it possible to identify YAC clones without a cumbersome verification step by colony hybridization and/or Southern blotting. Using this method, we assigned 39 mouse YAC clones to a mouse genetic map with 13 biallelic, polymorphic expressed sequence tags. This method provides a fast, reliable way to identify YAC clones with PCR-based sequence-tagged sites.

Original languageEnglish
Pages (from-to)63-68
Number of pages6
JournalGenetic Analysis Techniques and Applications
Volume11
Issue number3
DOIs
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

Yeast Artificial Chromosomes
Polymerase chain reaction
Chromosomes
Reaction products
Yeast
Digestion
Screening
Enzymes
Polymerase Chain Reaction
Clone Cells
Sequence Tagged Sites
Expressed Sequence Tags
Southern Blotting

ASJC Scopus subject areas

  • Genetics

Cite this

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