Simple and stable lipid bilayer formation: A droplets contacting method using parylene micro-pores for multiple ion channel recordings

Yutaro Tsuji, Ryuji Kawano, Toshihisa Osaki, Hirotaka Sasaki, Norihisa Miki, Shoji Takeuchi

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

This paper describes multiple ion-channel recordings through membrane proteins reconstituted in bilayer lipid membranes (BLMs) array. The BLMs array can be prepared by "Droplets Contacting Method" which forms BLMs at the interface of two lipid monolayers. Since this method does not require skilled techniques, it is highly reproducible and can be applied to automated system. We used a double well chip (DWC) for the droplets contacting method. We attempted to confine the BLMs forming areas with parylene micro-pore (parylene double well chip, PDWC) to augment the mechanical stability of BLMs. Subsequently, we arrayed the PDWC with electrodes for multiple recordings of channel proteins. We successfully demonstrated 14 channels simultaneous ion channel recordings through α-hemolysin.

Original languageEnglish
Pages (from-to)419-424
Number of pages6
Journalieej transactions on sensors and micromachines
Volume131
Issue number12
DOIs
Publication statusPublished - 2011

Keywords

  • Bilayer lipid membranes
  • Droplets Contacting Method
  • Drug screening
  • Membrane protein
  • Micro total analysis system

ASJC Scopus subject areas

  • Mechanical Engineering
  • Electrical and Electronic Engineering

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