Simple autogeneic feeder cell preparation for pluripotent stem cells

Weizhen Li, Hiromi Yamashita, Fumiyuki Hattori, Hao Chen, Shugo Tohyama, Yusuke Satoh, Erika Sasaki, Shinsuke Yuasa, Shinji Makino, Motoaki Sano, Keiichi Fukuda

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Mouse embryonic fibroblasts (MEFs) are the most commonly used feeder cells for pluripotent stem cells. However, autogeneic feeder (AF) cells have several advantages such as no xenogeneic risks and reduced costs. In this report, we demonstrate that common marmoset embryonic stem (cmES) cells can be maintained on common marmoset AF (cmAF) cells. These cmES cells were maintained on cmAF cells for 6. months, retaining their morphology, normal karyotype, and expression patterns for the pluripotent markers Oct-3/4, Nanog, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81, as well as their ability to differentiate into cardiac and neural cells. Antibody array analysis revealed equivalent protein expression profiles between cmES cells maintained on cmAF cells and MEFs. In addition, similarly prepared human embryonic stem (hES) and induced pluripotent stem (hiPS) cell-derived AF cells supported the growth of and maintained the morphology and pluripotent marker expressions of hES and hiPS cells, respectively. DNA microarray analysis revealed that these hES and hiPS cells had mRNA expression profiles similar to those of hES and hiPS cells maintained on MEFs, respectively. Taken together, these findings imply that AF cells can replace MEFs in the routine maintenance of primate pluripotent stem cells.

Original languageEnglish
Pages (from-to)83-89
Number of pages7
JournalStem Cell Research
Volume6
Issue number1
DOIs
Publication statusPublished - 2011 Jan 1

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ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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