SIMPLE binds specifically to PI4P through SIMPLE-like domain and participates in protein trafficking in the trans-Golgi network and/or recycling endosomes

Yasuhiro Moriwaki, Yuho Ohno, Tomohiro Ishii, Yuki Takamura, Yuko Kita, Kazuhiko Watabe, Kazunori Sango, Shoutaro Tsuji, Hidemi Misawa

Research output: Contribution to journalArticle

Abstract

Small integral membrane protein of the lysosome/late endosome (SIMPLE) is a 161-amino acid cellular protein that contains a characteristic C-terminal domain known as the SIMPLE-like domain (SLD), which is well conserved among species. Several studies have demonstrated that SIMPLE localizes to the trans-Golgi network (TGN), early endosomes, lysosomes, multivesicular bodies, aggresomes and the plasma membrane. However, the amino acid regions responsible for its subcellular localization have not yet been identified. The SLD resembles the FYVE domain, which binds phosphatidylinositol (3)-phosphate (PI3P) and determines the subcellular localization of FYVE domain-containing proteins. In the present study, we have found that SIMPLE binds specifically to PI4P through its SLD. SIMPLE colocalized with PI4P and Rab11, a marker for recycling endosomes (REs, organelles enriched in PI4P) in both the IMS32 mouse Schwann cell line and Hela cells. Sucrose density-gradient centrifugation revealed that SIMPLE co-fractionated with syntaxin-6 (a TGN marker) and Rab11. We have also found that SIMPLE knockdown impeded recycling of transferrin and of transferrin receptor. Our overall results indicate that SIMPLE may regulate protein trafficking physiologically by localizing to the TGN and/or REs by binding PI4P.

Original languageEnglish
Article numbere0199829
JournalPLoS One
Volume13
Issue number6
DOIs
Publication statusPublished - 2018 Jun 1

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trans-Golgi Network
endosomes
protein transport
Endosomes
lysosomes
Protein Transport
Lysosomes
membrane proteins
recycling
Recycling
Membrane Proteins
Proteins
transferrin
Qa-SNARE Proteins
Amino Acids
Transferrin Receptors
Centrifugation
Multivesicular Bodies
Cell membranes
Transferrin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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SIMPLE binds specifically to PI4P through SIMPLE-like domain and participates in protein trafficking in the trans-Golgi network and/or recycling endosomes. / Moriwaki, Yasuhiro; Ohno, Yuho; Ishii, Tomohiro; Takamura, Yuki; Kita, Yuko; Watabe, Kazuhiko; Sango, Kazunori; Tsuji, Shoutaro; Misawa, Hidemi.

In: PLoS One, Vol. 13, No. 6, e0199829, 01.06.2018.

Research output: Contribution to journalArticle

Moriwaki, Yasuhiro ; Ohno, Yuho ; Ishii, Tomohiro ; Takamura, Yuki ; Kita, Yuko ; Watabe, Kazuhiko ; Sango, Kazunori ; Tsuji, Shoutaro ; Misawa, Hidemi. / SIMPLE binds specifically to PI4P through SIMPLE-like domain and participates in protein trafficking in the trans-Golgi network and/or recycling endosomes. In: PLoS One. 2018 ; Vol. 13, No. 6.
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