Simultaneous administration of recombinant measles viruses expressing respiratory syncytial virus fusion (F) and nucleo (N) proteins induced humoral and cellular immune responses in cotton rats

Yoshiaki Yamaji, Akihito Sawada, Yosuke Yasui, Takashi Ito, Tetsuo Nakayama

Research output: Contribution to journalArticle

Abstract

We previously reported that recombinant measles virus expressing the respiratory syncytial virus (RSV) fusion protein (F), MVAIK/RSV/F, induced neutralizing antibodies against RSV, and those expressing RSV-NP (MVAIK/RSV/NP) and M2-1 (MVAIK/RSV/M2-1) induced RSV-specific CD8 + /IFN-γ + cells, but not neutralizing antibodies. In the present study, MVAIK/RSV/F and MVAIK/RSV/NP were simultaneously administered to cotton rats and immune responses and protective effects were compared with MVAIK/RSV/F alone. Sufficient neutralizing antibodies against RSV and RSV-specific CD8 + /IFN-γ + cells were observed after re-immunization with simultaneous administration. After the RSV challenge, CD8 + /IFN-γ + increased in spleen cells obtained from the simultaneous immunization group in response to F and NP peptides. Higher numbers of CD8 + /IFN-γ + and CD4 + /IFN-γ + cells were detected in lung tissues from the simultaneous immunization group after the RSV challenge. No detectable RSV was recovered from lung homogenates in the immunized groups. Mild inflammatory reactions with the thickening of broncho-epithelial cells and the infiltration of inflammatory cells were observed in lung tissues obtained from cotton rats immunized with MVAIK/RSV/F alone after the RSV challenge. No inflammatory responses were observed after the RSV challenge in the simultaneous immunization groups. The present results indicate that combined administration with MVAIK/RSV/F and MVAIK/RSV/NP induces humoral and cellular immune responses and shows effective protection against RSV, suggesting the importance of cellular immunity.

Original languageEnglish
Article number27
JournalVaccines
Volume7
Issue number1
DOIs
Publication statusPublished - 2019 Mar 1

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Sigmodontinae
Measles virus
Respiratory Syncytial Viruses
Humoral Immunity
Cellular Immunity
Proteins
Immunization
Neutralizing Antibodies
Lung

Keywords

  • Cotton rat
  • F
  • NP
  • Recombinant virus
  • RSV

ASJC Scopus subject areas

  • Immunology
  • Pharmacology
  • Drug Discovery
  • Infectious Diseases
  • Pharmacology (medical)

Cite this

Simultaneous administration of recombinant measles viruses expressing respiratory syncytial virus fusion (F) and nucleo (N) proteins induced humoral and cellular immune responses in cotton rats. / Yamaji, Yoshiaki; Sawada, Akihito; Yasui, Yosuke; Ito, Takashi; Nakayama, Tetsuo.

In: Vaccines, Vol. 7, No. 1, 27, 01.03.2019.

Research output: Contribution to journalArticle

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