Simultaneous analysis of propionic acid analgesic and antipyretic drugs in human plasma by gas chromatography

Tatsuo Shinozuka, Rika Nakajima, Setsuko Takei, Osamu Ohue, Akira Ogamo, Masaru Terada, Tatsuya Murai, Junichi Yanagida

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A detailed procedure for simultaneous determination of ten propionic acid analgesic and antipyretic drugs in human plasma has been developed using medium-bore capillary gas chromatography (GC) with flame ionization detection. The propionic acid drugs in human plasma were extracted with chloroform at pH 3.0, and further purified with a Bond Elut® (silica) column. Distinct peaks of methyl derivatives of ten propionic acid drugs could be detected simultaneously by GC after on-column methylation with phenyltrimethylammonium hydroxide. Calibration curves of the drug derivatives were linear at 5.0-25.0 mg/ml. The detection limits were 0.2-2.0 μg/ml of plasma. The present procedure seems useful as a convenient screening and determination method in forensic chemistry.

Original languageEnglish
Pages (from-to)51-60
Number of pages10
JournalJapanese Journal of Forensic Toxicology
Volume17
Issue number1
Publication statusPublished - 1999

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Plasma Gases
Antipyretics
Gas chromatography
Gas Chromatography
Analgesics
Plasma (human)
Pharmaceutical Preparations
Flame Ionization
Propionates
Chloroform
Derivatives
Silicon Dioxide
Methylation
Calibration
Limit of Detection
Ionization
Screening
Plasmas
propionic acid

Keywords

  • Analgesics
  • Antipyretics
  • Gas chromatography
  • Non-steroidal anti-inflammatory drugs
  • On-column methylation
  • Propionic acid derivatives

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Toxicology

Cite this

Simultaneous analysis of propionic acid analgesic and antipyretic drugs in human plasma by gas chromatography. / Shinozuka, Tatsuo; Nakajima, Rika; Takei, Setsuko; Ohue, Osamu; Ogamo, Akira; Terada, Masaru; Murai, Tatsuya; Yanagida, Junichi.

In: Japanese Journal of Forensic Toxicology, Vol. 17, No. 1, 1999, p. 51-60.

Research output: Contribution to journalArticle

Shinozuka, T, Nakajima, R, Takei, S, Ohue, O, Ogamo, A, Terada, M, Murai, T & Yanagida, J 1999, 'Simultaneous analysis of propionic acid analgesic and antipyretic drugs in human plasma by gas chromatography', Japanese Journal of Forensic Toxicology, vol. 17, no. 1, pp. 51-60.
Shinozuka, Tatsuo ; Nakajima, Rika ; Takei, Setsuko ; Ohue, Osamu ; Ogamo, Akira ; Terada, Masaru ; Murai, Tatsuya ; Yanagida, Junichi. / Simultaneous analysis of propionic acid analgesic and antipyretic drugs in human plasma by gas chromatography. In: Japanese Journal of Forensic Toxicology. 1999 ; Vol. 17, No. 1. pp. 51-60.
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