Simultaneous determination of 8 kinds of organic acids in formula feed by capillary electrophoresis

E. Ishikuro, H. Hibino, T. Soga, H. Yanai, H. Sawada

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

An analytical method for organic acids (formic acid, fumaric acid, tartaric acid, malic acid, citric acid, acetic acid, lactic acid and propionic acid) in formula feeds by capillary electrophoresis (CE) was established. Organic acids were simultaneously extracted with water. The extract was filtered, diluted with 0.02 mol/L NaOH, and deproteinized by an ultrafiltration filter (30 kDa cut-off). The recovery test was conducted with 3 kinds of formula feed spiked with 8 kinds of organic acids at levels of 0.05~2.0%. The mean recovery values were in the range of 85.1~107.3%, and the relative standard deviation of repeatability (RSD) was within 13.6%. A collaborative study (3 laboratories) was conducted with formula feed spiked with 8 kinds of organic acids at levels of 0.1~1.0%. The mean recovery was 87.3~105.0%, and the relative standard deviation of reproducibility (RSD(R)) was within 16.3%.

Original languageEnglish
Pages (from-to)261-267
Number of pages7
JournalJournal of the Food Hygienic Society of Japan
Volume41
Issue number4
DOIs
Publication statusPublished - 2000 Jan 1
Externally publishedYes

Keywords

  • Capillary electrophoresis
  • Collaborative study
  • Feed additive
  • Formula feed
  • Organic acid
  • Ultrafiltration filter

ASJC Scopus subject areas

  • Food Science
  • Public Health, Environmental and Occupational Health

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