Simultaneous determination of anionic intermediates for Bacillus subtilis metabolic pathways by capillary electrophoresis electrospray ionization mass spectrometry

Tomoyoshi Soga, Yuki Ueno, Hisako Naraoka, Yoshiaki Ohashi, Masaru Tomita, Takaaki Nishioka

Research output: Contribution to journalArticle

339 Citations (Scopus)

Abstract

A method for simultaneous determination of anionic metabolites based on capillary electrophoresis (CE) coupled to electrospray ionization mass spectrometry is described. To prevent current drop by the system, electroosmotic flow (EOF) reversal by using a cationic polymer-coated capillary was indispensable. A mixture containing 32 standards including carboxylic acids, phosphorylated carboxylic acids, phosphorylated saccharides, nucleotides, and nicotinamide and flavin adenine coenzymes of glycolysis and the tricarboxylic acid cycle pathways were separated by CE and selectively detected by a quadrupole mass spectrometer with a sheath-flow electrospray ionization interface. Key to the analysis was EOF reversal using a cationic polymer-coated capillary and an electrolyte system consisting of 50 mM ammonium acetate, pH 9.0. The relative standard deviations of the method were better than 0.4% for migration times and between 0.9% and 5.4% for peak areas. The concentration detection limits for these metabolites were between 0.3 and 6.7 μmol/L with pressure injection of 50 mbar for 30 s (30 nL); i.e., mass detection limits ranged from 9 to 200 fmol, at a signal-to-noise ratio of 3. This method was applied to the comprehensive analysis of metabolic intermediates extracted from Bacillus subtilis, and 27 anionic metabolites could be directly detected and quantified.

Original languageEnglish
Pages (from-to)2233-2239
Number of pages7
JournalAnalytical Chemistry
Volume74
Issue number10
DOIs
Publication statusPublished - 2002 May 15

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Capillary electrophoresis
Electrospray ionization
Bacilli
Metabolites
Mass spectrometry
Carboxylic Acids
Polymers
Niacinamide
Coenzymes
Mass spectrometers
Adenine
Electrolytes
Signal to noise ratio
Nucleotides
Metabolic Networks and Pathways

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Simultaneous determination of anionic intermediates for Bacillus subtilis metabolic pathways by capillary electrophoresis electrospray ionization mass spectrometry. / Soga, Tomoyoshi; Ueno, Yuki; Naraoka, Hisako; Ohashi, Yoshiaki; Tomita, Masaru; Nishioka, Takaaki.

In: Analytical Chemistry, Vol. 74, No. 10, 15.05.2002, p. 2233-2239.

Research output: Contribution to journalArticle

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