Simultaneous determination of catecholamines, their basic metabolites and serotonin in urine by high‐performance liquid chromatography using A mixed‐mode column and an eight‐channel electrochemical detector

Fumiko Mashige, Yoshikazu Matsushima, Chizuko Miyata, Rika Yamada, Hideko Kanazawa, Ichiro Sakuma, Nobuharu Takai, Noriko Shinozuka, Akiyuki Ohkubo, Kazuhiko Nakahara

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

A high‐performance liquid chromatographic method for simultaneous determination of free catecholamines, their basic metabolites and serotonin in human urine was developed. The compounds were separated on a precolumn of cation‐exchange resin and a mixed‐mode (C18/cation‐exchange) column and determined by an eight‐channel electrochemical detector. By this method, free norepinephrine (NE), epinephrine (E), dopamine (DA), normetanephrine (NMN), metanephrine (MN), 3‐methoxy‐tyramine (3‐MT) and serotonin (5‐HT) were determined in 40 min with inter‐assay precision of 1.9, 3.2, 1.2, 1.6, 1.6, 5.5 and 3.2%, respectively. Detection limits were between 0.05 (E) and 0.3 (3‐MT) pmol. The mean values of analytical recovery for NE, E, DA, NMN, MN, 3‐MT, 5‐HT were 99, 104, 104, 99, 102, 109 and 99% respectively.

Original languageEnglish
Pages (from-to)221-225
Number of pages5
JournalBiomedical Chromatography
Volume9
Issue number5
DOIs
Publication statusPublished - 1995

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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