TY - JOUR
T1 - Simultaneous determination of catecholamines, their basic metabolites and serotonin in urine by high‐performance liquid chromatography using A mixed‐mode column and an eight‐channel electrochemical detector
AU - Mashige, Fumiko
AU - Matsushima, Yoshikazu
AU - Miyata, Chizuko
AU - Yamada, Rika
AU - Kanazawa, Hideko
AU - Sakuma, Ichiro
AU - Takai, Nobuharu
AU - Shinozuka, Noriko
AU - Ohkubo, Akiyuki
AU - Nakahara, Kazuhiko
PY - 1995
Y1 - 1995
N2 - A high‐performance liquid chromatographic method for simultaneous determination of free catecholamines, their basic metabolites and serotonin in human urine was developed. The compounds were separated on a precolumn of cation‐exchange resin and a mixed‐mode (C18/cation‐exchange) column and determined by an eight‐channel electrochemical detector. By this method, free norepinephrine (NE), epinephrine (E), dopamine (DA), normetanephrine (NMN), metanephrine (MN), 3‐methoxy‐tyramine (3‐MT) and serotonin (5‐HT) were determined in 40 min with inter‐assay precision of 1.9, 3.2, 1.2, 1.6, 1.6, 5.5 and 3.2%, respectively. Detection limits were between 0.05 (E) and 0.3 (3‐MT) pmol. The mean values of analytical recovery for NE, E, DA, NMN, MN, 3‐MT, 5‐HT were 99, 104, 104, 99, 102, 109 and 99% respectively.
AB - A high‐performance liquid chromatographic method for simultaneous determination of free catecholamines, their basic metabolites and serotonin in human urine was developed. The compounds were separated on a precolumn of cation‐exchange resin and a mixed‐mode (C18/cation‐exchange) column and determined by an eight‐channel electrochemical detector. By this method, free norepinephrine (NE), epinephrine (E), dopamine (DA), normetanephrine (NMN), metanephrine (MN), 3‐methoxy‐tyramine (3‐MT) and serotonin (5‐HT) were determined in 40 min with inter‐assay precision of 1.9, 3.2, 1.2, 1.6, 1.6, 5.5 and 3.2%, respectively. Detection limits were between 0.05 (E) and 0.3 (3‐MT) pmol. The mean values of analytical recovery for NE, E, DA, NMN, MN, 3‐MT, 5‐HT were 99, 104, 104, 99, 102, 109 and 99% respectively.
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U2 - 10.1002/bmc.1130090506
DO - 10.1002/bmc.1130090506
M3 - Article
C2 - 8593422
AN - SCOPUS:0028850145
SN - 0269-3879
VL - 9
SP - 221
EP - 225
JO - Biomedical Chromatography
JF - Biomedical Chromatography
IS - 5
ER -