Simultaneous determination of flunitrazepam and 7-aminoflunitrazepam in human serum by ion trap gas chromatography-tandem mass spectrometry

Masaru Terada, Sousuke Masui, Takeshi Hayashi, Ritsuko Watanabe, Hiromasa Inoue, Morio Iino, Masato Nakatome, Ryoji Matoba, Tatsuo Shinozuka, Tatsuya Murai, Einosuke Tanaka, Katsuya Honda

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

A method for the determination of flunitrazepam (FNZ) and 7-aminoflunitrazepam (7-AFNZ) in human serum was developed with ion trap gas chromatography (GC)-tandem mass spectrometry. The 7-AFNZ was derivatizated with 50 μ1 trifluoroacetic anhydride (TFAA), 60°C-20 min. EI mass spectra and tandem mass spectra of FNZ and 7-ANFZ-TFA were m/z 238, 239, 266, 286, 294, 312, 313(M+), m/z 350, 351, 360, 378, 379(M+), m/z 238, 239, 240 (precursor ion m/z 286, collision energy 1.5 V), and m/z 239, 254, 264, 336 (precursor ion m/z 351, collision energy 1.8 V), respectively. The detection limits of full scan EI mass spectrometry and tandem mass spectrometry for FNZ and 7-AFNZ in human serum were ca. 200 ng/ml, 60 ng/ml, 15 ng/ml and 1 ng/ml, respectively.

Original languageEnglish
Pages (from-to)S96-S100
JournalLegal Medicine
Volume5
Issue numberSUPPL. 1
DOIs
Publication statusPublished - 2003

Keywords

  • 7-aminoflunitrazepam
  • Flunitrazepam
  • GC-MS/MS
  • Human serum
  • Ion trap gas chromatography-tandem mass spectrometry
  • Trifluoroacetyl derivative

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Issues, ethics and legal aspects

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