Simultaneous determination of flunitrazepam and 7-aminoflunitrazepam in human serum by ion trap gas chromatography-tandem mass spectrometry

Masaru Terada, Sousuke Masui, Takeshi Hayashi, Ritsuko Watanabe, Hiromasa Inoue, Morio Iino, Masato Nakatome, Ryoji Matoba, Tatsuo Shinozuka, Tatsuya Murai, Einosuke Tanaka, Katsuya Honda

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A method for the determination of flunitrazepam (FNZ) and 7-aminoflunitrazepam (7-AFNZ) in human serum was developed with ion trap gas chromatography (GC)-tandem mass spectrometry. The 7-AFNZ was derivatizated with 50 μ1 trifluoroacetic anhydride (TFAA), 60°C-20 min. EI mass spectra and tandem mass spectra of FNZ and 7-ANFZ-TFA were m/z 238, 239, 266, 286, 294, 312, 313(M+), m/z 350, 351, 360, 378, 379(M+), m/z 238, 239, 240 (precursor ion m/z 286, collision energy 1.5 V), and m/z 239, 254, 264, 336 (precursor ion m/z 351, collision energy 1.8 V), respectively. The detection limits of full scan EI mass spectrometry and tandem mass spectrometry for FNZ and 7-AFNZ in human serum were ca. 200 ng/ml, 60 ng/ml, 15 ng/ml and 1 ng/ml, respectively.

Original languageEnglish
JournalLegal Medicine
Volume5
Issue numberSUPPL. 1
DOIs
Publication statusPublished - 2003
Externally publishedYes

Fingerprint

Flunitrazepam
Tandem Mass Spectrometry
Gas Chromatography-Mass Spectrometry
trifluoroacetic anhydride
Ions
Serum
Limit of Detection
7-aminoflunitrazepam

Keywords

  • 7-aminoflunitrazepam
  • Flunitrazepam
  • GC-MS/MS
  • Human serum
  • Ion trap gas chromatography-tandem mass spectrometry
  • Trifluoroacetyl derivative

ASJC Scopus subject areas

  • Genetics
  • Pathology and Forensic Medicine
  • Psychiatry and Mental health
  • Public Health, Environmental and Occupational Health

Cite this

Simultaneous determination of flunitrazepam and 7-aminoflunitrazepam in human serum by ion trap gas chromatography-tandem mass spectrometry. / Terada, Masaru; Masui, Sousuke; Hayashi, Takeshi; Watanabe, Ritsuko; Inoue, Hiromasa; Iino, Morio; Nakatome, Masato; Matoba, Ryoji; Shinozuka, Tatsuo; Murai, Tatsuya; Tanaka, Einosuke; Honda, Katsuya.

In: Legal Medicine, Vol. 5, No. SUPPL. 1, 2003.

Research output: Contribution to journalArticle

Terada, M, Masui, S, Hayashi, T, Watanabe, R, Inoue, H, Iino, M, Nakatome, M, Matoba, R, Shinozuka, T, Murai, T, Tanaka, E & Honda, K 2003, 'Simultaneous determination of flunitrazepam and 7-aminoflunitrazepam in human serum by ion trap gas chromatography-tandem mass spectrometry', Legal Medicine, vol. 5, no. SUPPL. 1. https://doi.org/10.1016/S1344-6223(02)00075-5
Terada, Masaru ; Masui, Sousuke ; Hayashi, Takeshi ; Watanabe, Ritsuko ; Inoue, Hiromasa ; Iino, Morio ; Nakatome, Masato ; Matoba, Ryoji ; Shinozuka, Tatsuo ; Murai, Tatsuya ; Tanaka, Einosuke ; Honda, Katsuya. / Simultaneous determination of flunitrazepam and 7-aminoflunitrazepam in human serum by ion trap gas chromatography-tandem mass spectrometry. In: Legal Medicine. 2003 ; Vol. 5, No. SUPPL. 1.
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abstract = "A method for the determination of flunitrazepam (FNZ) and 7-aminoflunitrazepam (7-AFNZ) in human serum was developed with ion trap gas chromatography (GC)-tandem mass spectrometry. The 7-AFNZ was derivatizated with 50 μ1 trifluoroacetic anhydride (TFAA), 60°C-20 min. EI mass spectra and tandem mass spectra of FNZ and 7-ANFZ-TFA were m/z 238, 239, 266, 286, 294, 312, 313(M+), m/z 350, 351, 360, 378, 379(M+), m/z 238, 239, 240 (precursor ion m/z 286, collision energy 1.5 V), and m/z 239, 254, 264, 336 (precursor ion m/z 351, collision energy 1.8 V), respectively. The detection limits of full scan EI mass spectrometry and tandem mass spectrometry for FNZ and 7-AFNZ in human serum were ca. 200 ng/ml, 60 ng/ml, 15 ng/ml and 1 ng/ml, respectively.",
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AU - Watanabe, Ritsuko

AU - Inoue, Hiromasa

AU - Iino, Morio

AU - Nakatome, Masato

AU - Matoba, Ryoji

AU - Shinozuka, Tatsuo

AU - Murai, Tatsuya

AU - Tanaka, Einosuke

AU - Honda, Katsuya

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N2 - A method for the determination of flunitrazepam (FNZ) and 7-aminoflunitrazepam (7-AFNZ) in human serum was developed with ion trap gas chromatography (GC)-tandem mass spectrometry. The 7-AFNZ was derivatizated with 50 μ1 trifluoroacetic anhydride (TFAA), 60°C-20 min. EI mass spectra and tandem mass spectra of FNZ and 7-ANFZ-TFA were m/z 238, 239, 266, 286, 294, 312, 313(M+), m/z 350, 351, 360, 378, 379(M+), m/z 238, 239, 240 (precursor ion m/z 286, collision energy 1.5 V), and m/z 239, 254, 264, 336 (precursor ion m/z 351, collision energy 1.8 V), respectively. The detection limits of full scan EI mass spectrometry and tandem mass spectrometry for FNZ and 7-AFNZ in human serum were ca. 200 ng/ml, 60 ng/ml, 15 ng/ml and 1 ng/ml, respectively.

AB - A method for the determination of flunitrazepam (FNZ) and 7-aminoflunitrazepam (7-AFNZ) in human serum was developed with ion trap gas chromatography (GC)-tandem mass spectrometry. The 7-AFNZ was derivatizated with 50 μ1 trifluoroacetic anhydride (TFAA), 60°C-20 min. EI mass spectra and tandem mass spectra of FNZ and 7-ANFZ-TFA were m/z 238, 239, 266, 286, 294, 312, 313(M+), m/z 350, 351, 360, 378, 379(M+), m/z 238, 239, 240 (precursor ion m/z 286, collision energy 1.5 V), and m/z 239, 254, 264, 336 (precursor ion m/z 351, collision energy 1.8 V), respectively. The detection limits of full scan EI mass spectrometry and tandem mass spectrometry for FNZ and 7-AFNZ in human serum were ca. 200 ng/ml, 60 ng/ml, 15 ng/ml and 1 ng/ml, respectively.

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