Simultaneous determination of flunitrazepam and 7-aminoflunitrazepam in human serum by ion trap gas chromatography-tandem mass spectrometry

Masaru Terada; Sousuke Masui; Takeshi Hayashi; Ritsuko Watanabe; Hiromasa Inoue; Morio Iino; Masato Nakatome; Ryoji Matoba; Tatsuo Shinozuka; Tatsuya Murai; Einosuke Tanaka; Katsuya Honda

doi:10.1016/S1344-6223(02)00075-5

Simultaneous determination of flunitrazepam and 7-aminoflunitrazepam in human serum by ion trap gas chromatography-tandem mass spectrometry

Masaru Terada, Sousuke Masui, Takeshi Hayashi, Ritsuko Watanabe, Hiromasa Inoue, Morio Iino, Masato Nakatome, Ryoji Matoba, Tatsuo Shinozuka, Tatsuya Murai, Einosuke Tanaka, Katsuya Honda

Department of Legal Medicine

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

A method for the determination of flunitrazepam (FNZ) and 7-aminoflunitrazepam (7-AFNZ) in human serum was developed with ion trap gas chromatography (GC)-tandem mass spectrometry. The 7-AFNZ was derivatizated with 50 μ1 trifluoroacetic anhydride (TFAA), 60°C-20 min. EI mass spectra and tandem mass spectra of FNZ and 7-ANFZ-TFA were m/z 238, 239, 266, 286, 294, 312, 313(M⁺), m/z 350, 351, 360, 378, 379(M⁺), m/z 238, 239, 240 (precursor ion m/z 286, collision energy 1.5 V), and m/z 239, 254, 264, 336 (precursor ion m/z 351, collision energy 1.8 V), respectively. The detection limits of full scan EI mass spectrometry and tandem mass spectrometry for FNZ and 7-AFNZ in human serum were ca. 200 ng/ml, 60 ng/ml, 15 ng/ml and 1 ng/ml, respectively.

Original language	English
Pages (from-to)	S96-S100
Journal	Legal Medicine
Volume	5
Issue number	SUPPL. 1
DOIs	https://doi.org/10.1016/S1344-6223(02)00075-5
Publication status	Published - 2003

Keywords

7-aminoflunitrazepam
Flunitrazepam
GC-MS/MS
Human serum
Ion trap gas chromatography-tandem mass spectrometry
Trifluoroacetyl derivative

ASJC Scopus subject areas

Pathology and Forensic Medicine
Issues, ethics and legal aspects

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S1344-6223(02)00075-5

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Terada, M., Masui, S., Hayashi, T., Watanabe, R., Inoue, H., Iino, M., Nakatome, M., Matoba, R., Shinozuka, T., Murai, T., Tanaka, E., & Honda, K. (2003). Simultaneous determination of flunitrazepam and 7-aminoflunitrazepam in human serum by ion trap gas chromatography-tandem mass spectrometry. Legal Medicine, 5(SUPPL. 1), S96-S100. https://doi.org/10.1016/S1344-6223(02)00075-5

Terada, M, Masui, S, Hayashi, T, Watanabe, R, Inoue, H, Iino, M, Nakatome, M, Matoba, R, Shinozuka, T, Murai, T, Tanaka, E & Honda, K 2003, 'Simultaneous determination of flunitrazepam and 7-aminoflunitrazepam in human serum by ion trap gas chromatography-tandem mass spectrometry', Legal Medicine, vol. 5, no. SUPPL. 1, pp. S96-S100. https://doi.org/10.1016/S1344-6223(02)00075-5

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title = "Simultaneous determination of flunitrazepam and 7-aminoflunitrazepam in human serum by ion trap gas chromatography-tandem mass spectrometry",

abstract = "A method for the determination of flunitrazepam (FNZ) and 7-aminoflunitrazepam (7-AFNZ) in human serum was developed with ion trap gas chromatography (GC)-tandem mass spectrometry. The 7-AFNZ was derivatizated with 50 μ1 trifluoroacetic anhydride (TFAA), 60°C-20 min. EI mass spectra and tandem mass spectra of FNZ and 7-ANFZ-TFA were m/z 238, 239, 266, 286, 294, 312, 313(M+), m/z 350, 351, 360, 378, 379(M+), m/z 238, 239, 240 (precursor ion m/z 286, collision energy 1.5 V), and m/z 239, 254, 264, 336 (precursor ion m/z 351, collision energy 1.8 V), respectively. The detection limits of full scan EI mass spectrometry and tandem mass spectrometry for FNZ and 7-AFNZ in human serum were ca. 200 ng/ml, 60 ng/ml, 15 ng/ml and 1 ng/ml, respectively.",

keywords = "7-aminoflunitrazepam, Flunitrazepam, GC-MS/MS, Human serum, Ion trap gas chromatography-tandem mass spectrometry, Trifluoroacetyl derivative",

author = "Masaru Terada and Sousuke Masui and Takeshi Hayashi and Ritsuko Watanabe and Hiromasa Inoue and Morio Iino and Masato Nakatome and Ryoji Matoba and Tatsuo Shinozuka and Tatsuya Murai and Einosuke Tanaka and Katsuya Honda",

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doi = "10.1016/S1344-6223(02)00075-5",

language = "English",

volume = "5",

pages = "S96--S100",

journal = "Legal Medicine",

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publisher = "Elsevier",

number = "SUPPL. 1",

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T1 - Simultaneous determination of flunitrazepam and 7-aminoflunitrazepam in human serum by ion trap gas chromatography-tandem mass spectrometry

AU - Terada, Masaru

AU - Masui, Sousuke

AU - Hayashi, Takeshi

AU - Watanabe, Ritsuko

AU - Inoue, Hiromasa

AU - Iino, Morio

AU - Nakatome, Masato

AU - Matoba, Ryoji

AU - Shinozuka, Tatsuo

AU - Murai, Tatsuya

AU - Tanaka, Einosuke

AU - Honda, Katsuya

PY - 2003

Y1 - 2003

N2 - A method for the determination of flunitrazepam (FNZ) and 7-aminoflunitrazepam (7-AFNZ) in human serum was developed with ion trap gas chromatography (GC)-tandem mass spectrometry. The 7-AFNZ was derivatizated with 50 μ1 trifluoroacetic anhydride (TFAA), 60°C-20 min. EI mass spectra and tandem mass spectra of FNZ and 7-ANFZ-TFA were m/z 238, 239, 266, 286, 294, 312, 313(M+), m/z 350, 351, 360, 378, 379(M+), m/z 238, 239, 240 (precursor ion m/z 286, collision energy 1.5 V), and m/z 239, 254, 264, 336 (precursor ion m/z 351, collision energy 1.8 V), respectively. The detection limits of full scan EI mass spectrometry and tandem mass spectrometry for FNZ and 7-AFNZ in human serum were ca. 200 ng/ml, 60 ng/ml, 15 ng/ml and 1 ng/ml, respectively.

AB - A method for the determination of flunitrazepam (FNZ) and 7-aminoflunitrazepam (7-AFNZ) in human serum was developed with ion trap gas chromatography (GC)-tandem mass spectrometry. The 7-AFNZ was derivatizated with 50 μ1 trifluoroacetic anhydride (TFAA), 60°C-20 min. EI mass spectra and tandem mass spectra of FNZ and 7-ANFZ-TFA were m/z 238, 239, 266, 286, 294, 312, 313(M+), m/z 350, 351, 360, 378, 379(M+), m/z 238, 239, 240 (precursor ion m/z 286, collision energy 1.5 V), and m/z 239, 254, 264, 336 (precursor ion m/z 351, collision energy 1.8 V), respectively. The detection limits of full scan EI mass spectrometry and tandem mass spectrometry for FNZ and 7-AFNZ in human serum were ca. 200 ng/ml, 60 ng/ml, 15 ng/ml and 1 ng/ml, respectively.

KW - 7-aminoflunitrazepam

KW - Flunitrazepam

KW - GC-MS/MS

KW - Human serum

KW - Ion trap gas chromatography-tandem mass spectrometry

KW - Trifluoroacetyl derivative

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AN - SCOPUS:0038077564

SN - 1344-6223

VL - 5

SP - S96-S100

JO - Legal Medicine

JF - Legal Medicine

IS - SUPPL. 1

ER -

Simultaneous determination of flunitrazepam and 7-aminoflunitrazepam in human serum by ion trap gas chromatography-tandem mass spectrometry

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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