A method for the determination of flunitrazepam (FNZ) and 7-aminoflunitrazepam (7-AFNZ) in human serum was developed with ion trap gas chromatography (GC)-tandem mass spectrometry. The 7-AFNZ was derivatizated with 50 μ1 trifluoroacetic anhydride (TFAA), 60°C-20 min. EI mass spectra and tandem mass spectra of FNZ and 7-ANFZ-TFA were m/z 238, 239, 266, 286, 294, 312, 313(M+), m/z 350, 351, 360, 378, 379(M+), m/z 238, 239, 240 (precursor ion m/z 286, collision energy 1.5 V), and m/z 239, 254, 264, 336 (precursor ion m/z 351, collision energy 1.8 V), respectively. The detection limits of full scan EI mass spectrometry and tandem mass spectrometry for FNZ and 7-AFNZ in human serum were ca. 200 ng/ml, 60 ng/ml, 15 ng/ml and 1 ng/ml, respectively.
- Human serum
- Ion trap gas chromatography-tandem mass spectrometry
- Trifluoroacetyl derivative
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Issues, ethics and legal aspects