Abstract
Octadecyl porous glass was used as the packing for reversed-phase high-performance liquid chromatography. A mixture of ginsenosides and saikosaponins (saponins of ginseng and bupleurum root, respectively) were analysed with detection at 203 nm. A well resolved chromatogram of ginsenoside Rb1, Rc, Rb2 and Rd and saikosaponin a, b2 and c was obtained with acetonitrile-water (25.5:74.5) as the mobile phase. The whole separation was achieved in 20 min with a flow-rate of 1.5 ml/min. Calibration graphs for ginsenoside Rb1, Rc, Rb2 and Rd and saisakosaponin a and c were linear up to 5 μg. Rapid and accurate simultaneous determinations of the saponins are possible by the described method.
| Original language | English |
|---|---|
| Pages (from-to) | 327-332 |
| Number of pages | 6 |
| Journal | Journal of Chromatography A |
| Volume | 507 |
| Issue number | C |
| DOIs | |
| Publication status | Published - 1990 May 16 |
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Organic Chemistry
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Cite this
Kanazawa, H., Nagata, Y., Matsushima, Y., Tomoda, M., & Takai, N. (1990). Simultaneous determination of ginsenosides and saikosaponins by high-performance liquid chromatography. Journal of Chromatography A, 507(C), 327-332. https://doi.org/10.1016/S0021-9673(01)84210-2