TY - JOUR
T1 - Simultaneous determination of ginsenosides and saikosaponins by high-performance liquid chromatography
AU - Kanazawa, Hideko
AU - Nagata, Yoshiko
AU - Matsushima, Yoshikazu
AU - Tomoda, Masashi
AU - Takai, Nobuharu
PY - 1990/5/16
Y1 - 1990/5/16
N2 - Octadecyl porous glass was used as the packing for reversed-phase high-performance liquid chromatography. A mixture of ginsenosides and saikosaponins (saponins of ginseng and bupleurum root, respectively) were analysed with detection at 203 nm. A well resolved chromatogram of ginsenoside Rb1, Rc, Rb2 and Rd and saikosaponin a, b2 and c was obtained with acetonitrile-water (25.5:74.5) as the mobile phase. The whole separation was achieved in 20 min with a flow-rate of 1.5 ml/min. Calibration graphs for ginsenoside Rb1, Rc, Rb2 and Rd and saisakosaponin a and c were linear up to 5 μg. Rapid and accurate simultaneous determinations of the saponins are possible by the described method.
AB - Octadecyl porous glass was used as the packing for reversed-phase high-performance liquid chromatography. A mixture of ginsenosides and saikosaponins (saponins of ginseng and bupleurum root, respectively) were analysed with detection at 203 nm. A well resolved chromatogram of ginsenoside Rb1, Rc, Rb2 and Rd and saikosaponin a, b2 and c was obtained with acetonitrile-water (25.5:74.5) as the mobile phase. The whole separation was achieved in 20 min with a flow-rate of 1.5 ml/min. Calibration graphs for ginsenoside Rb1, Rc, Rb2 and Rd and saisakosaponin a and c were linear up to 5 μg. Rapid and accurate simultaneous determinations of the saponins are possible by the described method.
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U2 - 10.1016/S0021-9673(01)84210-2
DO - 10.1016/S0021-9673(01)84210-2
M3 - Article
C2 - 2380300
AN - SCOPUS:0025249814
VL - 507
SP - 327
EP - 332
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - C
ER -