Abstract
Octadecyl porous glass was used as the packing for reversed-phase high-performance liquid chromatography. A mixture of ginsenosides and saikosaponins (saponins of ginseng and bupleurum root, respectively) were analysed with detection at 203 nm. A well resolved chromatogram of ginsenoside Rb1, Rc, Rb2 and Rd and saikosaponin a, b2 and c was obtained with acetonitrile-water (25.5:74.5) as the mobile phase. The whole separation was achieved in 20 min with a flow-rate of 1.5 ml/min. Calibration graphs for ginsenoside Rb1, Rc, Rb2 and Rd and saisakosaponin a and c were linear up to 5 μg. Rapid and accurate simultaneous determinations of the saponins are possible by the described method.
| Original language | English |
|---|---|
| Pages (from-to) | 327-332 |
| Number of pages | 6 |
| Journal | Journal of Chromatography A |
| Volume | 507 |
| Issue number | C |
| DOIs | |
| Publication status | Published - 1990 May 16 |
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ASJC Scopus subject areas
- Analytical Chemistry
- Clinical Biochemistry
- Molecular Medicine
Cite this
Simultaneous determination of ginsenosides and saikosaponins by high-performance liquid chromatography. / Kanazawa, Hideko; Itoh, Yoshiko; Matsushima, Yoshikazu; Tomoda, Masashi; Takai, Nobuharu.
In: Journal of Chromatography A, Vol. 507, No. C, 16.05.1990, p. 327-332.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Simultaneous determination of ginsenosides and saikosaponins by high-performance liquid chromatography
AU - Kanazawa, Hideko
AU - Itoh, Yoshiko
AU - Matsushima, Yoshikazu
AU - Tomoda, Masashi
AU - Takai, Nobuharu
PY - 1990/5/16
Y1 - 1990/5/16
N2 - Octadecyl porous glass was used as the packing for reversed-phase high-performance liquid chromatography. A mixture of ginsenosides and saikosaponins (saponins of ginseng and bupleurum root, respectively) were analysed with detection at 203 nm. A well resolved chromatogram of ginsenoside Rb1, Rc, Rb2 and Rd and saikosaponin a, b2 and c was obtained with acetonitrile-water (25.5:74.5) as the mobile phase. The whole separation was achieved in 20 min with a flow-rate of 1.5 ml/min. Calibration graphs for ginsenoside Rb1, Rc, Rb2 and Rd and saisakosaponin a and c were linear up to 5 μg. Rapid and accurate simultaneous determinations of the saponins are possible by the described method.
AB - Octadecyl porous glass was used as the packing for reversed-phase high-performance liquid chromatography. A mixture of ginsenosides and saikosaponins (saponins of ginseng and bupleurum root, respectively) were analysed with detection at 203 nm. A well resolved chromatogram of ginsenoside Rb1, Rc, Rb2 and Rd and saikosaponin a, b2 and c was obtained with acetonitrile-water (25.5:74.5) as the mobile phase. The whole separation was achieved in 20 min with a flow-rate of 1.5 ml/min. Calibration graphs for ginsenoside Rb1, Rc, Rb2 and Rd and saisakosaponin a and c were linear up to 5 μg. Rapid and accurate simultaneous determinations of the saponins are possible by the described method.
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UR - http://www.scopus.com/inward/citedby.url?scp=0025249814&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(01)84210-2
DO - 10.1016/S0021-9673(01)84210-2
M3 - Article
C2 - 2380300
AN - SCOPUS:0025249814
VL - 507
SP - 327
EP - 332
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0021-9673
IS - C
ER -