Simultaneous determination of ginsenosides and saikosaponins by high-performance liquid chromatography

Hideko Kanazawa; Yoshiko Nagata; Yoshikazu Matsushima; Masashi Tomoda; Nobuharu Takai

doi:10.1016/S0021-9673(01)84210-2

Simultaneous determination of ginsenosides and saikosaponins by high-performance liquid chromatography

Hideko Kanazawa, Yoshiko Nagata, Yoshikazu Matsushima, Masashi Tomoda, Nobuharu Takai

School of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

Octadecyl porous glass was used as the packing for reversed-phase high-performance liquid chromatography. A mixture of ginsenosides and saikosaponins (saponins of ginseng and bupleurum root, respectively) were analysed with detection at 203 nm. A well resolved chromatogram of ginsenoside Rb₁, Rc, Rb₂ and Rd and saikosaponin a, b₂ and c was obtained with acetonitrile-water (25.5:74.5) as the mobile phase. The whole separation was achieved in 20 min with a flow-rate of 1.5 ml/min. Calibration graphs for ginsenoside Rb₁, Rc, Rb₂ and Rd and saisakosaponin a and c were linear up to 5 μg. Rapid and accurate simultaneous determinations of the saponins are possible by the described method.

Original language	English
Pages (from-to)	327-332
Number of pages	6
Journal	Journal of Chromatography A
Volume	507
Issue number	C
DOIs	https://doi.org/10.1016/S0021-9673(01)84210-2
Publication status	Published - 1990 May 16

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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AU - Kanazawa, Hideko

AU - Nagata, Yoshiko

AU - Matsushima, Yoshikazu

AU - Tomoda, Masashi

AU - Takai, Nobuharu

PY - 1990/5/16

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N2 - Octadecyl porous glass was used as the packing for reversed-phase high-performance liquid chromatography. A mixture of ginsenosides and saikosaponins (saponins of ginseng and bupleurum root, respectively) were analysed with detection at 203 nm. A well resolved chromatogram of ginsenoside Rb1, Rc, Rb2 and Rd and saikosaponin a, b2 and c was obtained with acetonitrile-water (25.5:74.5) as the mobile phase. The whole separation was achieved in 20 min with a flow-rate of 1.5 ml/min. Calibration graphs for ginsenoside Rb1, Rc, Rb2 and Rd and saisakosaponin a and c were linear up to 5 μg. Rapid and accurate simultaneous determinations of the saponins are possible by the described method.

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