Simultaneous determination of ginsenosides and saikosaponins by high-performance liquid chromatography

Hideko Kanazawa, Yoshiko Nagata, Yoshikazu Matsushima, Masashi Tomoda, Nobuharu Takai

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Octadecyl porous glass was used as the packing for reversed-phase high-performance liquid chromatography. A mixture of ginsenosides and saikosaponins (saponins of ginseng and bupleurum root, respectively) were analysed with detection at 203 nm. A well resolved chromatogram of ginsenoside Rb1, Rc, Rb2 and Rd and saikosaponin a, b2 and c was obtained with acetonitrile-water (25.5:74.5) as the mobile phase. The whole separation was achieved in 20 min with a flow-rate of 1.5 ml/min. Calibration graphs for ginsenoside Rb1, Rc, Rb2 and Rd and saisakosaponin a and c were linear up to 5 μg. Rapid and accurate simultaneous determinations of the saponins are possible by the described method.

Original languageEnglish
Pages (from-to)327-332
Number of pages6
JournalJournal of Chromatography A
Issue numberC
Publication statusPublished - 1990 May 16


ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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