Simultaneous determination of ginsenosides and saikosaponins by high-performance liquid chromatography

Hideko Kanazawa, Yoshiko Itoh, Yoshikazu Matsushima, Masashi Tomoda, Nobuharu Takai

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Octadecyl porous glass was used as the packing for reversed-phase high-performance liquid chromatography. A mixture of ginsenosides and saikosaponins (saponins of ginseng and bupleurum root, respectively) were analysed with detection at 203 nm. A well resolved chromatogram of ginsenoside Rb1, Rc, Rb2 and Rd and saikosaponin a, b2 and c was obtained with acetonitrile-water (25.5:74.5) as the mobile phase. The whole separation was achieved in 20 min with a flow-rate of 1.5 ml/min. Calibration graphs for ginsenoside Rb1, Rc, Rb2 and Rd and saisakosaponin a and c were linear up to 5 μg. Rapid and accurate simultaneous determinations of the saponins are possible by the described method.

Original languageEnglish
Pages (from-to)327-332
Number of pages6
JournalJournal of Chromatography A
Volume507
Issue numberC
DOIs
Publication statusPublished - 1990 May 16

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Ginsenosides
Saponins
High performance liquid chromatography
Bupleurum
High Pressure Liquid Chromatography
Panax
Reverse-Phase Chromatography
Calibration
Glass
Flow rate
Water
ginsenoside Rb1
ginsenoside Rc
ginsenoside Rd
saikosaponin
acetonitrile

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Simultaneous determination of ginsenosides and saikosaponins by high-performance liquid chromatography. / Kanazawa, Hideko; Itoh, Yoshiko; Matsushima, Yoshikazu; Tomoda, Masashi; Takai, Nobuharu.

In: Journal of Chromatography A, Vol. 507, No. C, 16.05.1990, p. 327-332.

Research output: Contribution to journalArticle

Kanazawa, Hideko ; Itoh, Yoshiko ; Matsushima, Yoshikazu ; Tomoda, Masashi ; Takai, Nobuharu. / Simultaneous determination of ginsenosides and saikosaponins by high-performance liquid chromatography. In: Journal of Chromatography A. 1990 ; Vol. 507, No. C. pp. 327-332.
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