Simultaneous determination of theophylline and its metabolites by HPLC

Hideko Kanazawa, J. Kizu, Y. Matsushima

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A high performance liquid chromatography (HPLC) method has been developed for the simultaneous determination of theophylline and its metabolites, with caffeine and its metabolites. The method is simple and applicable to planning the administration of theophylline. We used a column-switching system in combination with atmospheric pressure chemical ionization liquid chromatography-mass spectrometry (LC-APCI-MS) for the determination of theophylline and its metabolites in biological samples. In the mass spectrum, the molecular ions of these drugs and metabolites were clearly observed as base peaks. The method is sufficiently sensitive and accurate for the pharmacokinetic studies of these drugs.

Original languageEnglish
Pages (from-to)1051-1060
Number of pages10
JournalYakugaku Zasshi
Volume120
Issue number10
Publication statusPublished - 2000

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Theophylline
High Pressure Liquid Chromatography
Atmospheric Pressure
Caffeine
Liquid Chromatography
Pharmaceutical Preparations
Mass Spectrometry
Pharmacokinetics
Ions

Keywords

  • Caffeine
  • Column-switching system
  • HPLC
  • LC/MS
  • Metabolite
  • Theophylline

ASJC Scopus subject areas

  • Molecular Medicine

Cite this

Simultaneous determination of theophylline and its metabolites by HPLC. / Kanazawa, Hideko; Kizu, J.; Matsushima, Y.

In: Yakugaku Zasshi, Vol. 120, No. 10, 2000, p. 1051-1060.

Research output: Contribution to journalArticle

Kanazawa, H, Kizu, J & Matsushima, Y 2000, 'Simultaneous determination of theophylline and its metabolites by HPLC', Yakugaku Zasshi, vol. 120, no. 10, pp. 1051-1060.
Kanazawa, Hideko ; Kizu, J. ; Matsushima, Y. / Simultaneous determination of theophylline and its metabolites by HPLC. In: Yakugaku Zasshi. 2000 ; Vol. 120, No. 10. pp. 1051-1060.
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