Simultaneous measurement of atrial natriuretic polypeptide (ANP) messenger RNA and ANP in rat heart - Evidence for a preferentially increased synthesis and secretion of ANP in left atrium of spontaneously hypertensive rats (SHR) -

Hiroshi Arai, Kazuwa Nakao, Yoshihiko Saito, Narito Morii, Akira Sugawara, Takayuki Yamada, Hiroshi Itoh, Shozo Shiono, Masashi Mukoyama, Hiroaki Ohkubo, Shigetada Nakanishi, Hiroo Imura

Research output: Contribution to journalArticle

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Abstract

Tissue levels of atrial natriuretic polypeptide (ANP) messenger RNA (ANPmRNA) and ANP in the rat heart were measured simultaneously. In Wistar rats, ANPmRNA of the same size (approximately 0.95 kbp) was detected in all four chambers of the rat heart. The ANPmRNA level was the highest in the right atrium, and the left atrial level was slightly lower than the right atrial level. Ventricular levels were more than two orders of magnitude lower than atrial levels. Tissue ANP concentrations of four chambers were roughly parallel to ANPmRNA levels. In spontaneously hypertensive rats (SHR) with the elevated plasma ANP level, the ANPmRNA level in the left atrium was substantially increased. The left/right ratio of atrial ANPmRNA level in SHR (150%) was higher than that in control Wistar Kyoto rats (WKY) (90%). In contrast, the left/right ratio of atrial ANP concentration was decreased in SHR (44%) compared with that in WKY (84%). The ratio of ANP to ANPmRNA levels in the left atrium of SHR was about three times smaller than that in the right atrium of SHR, and those in bilateral atria of WKY. These results indicate that the biosynthesis and secretion of ANP from the left atrium is preferentially increased in SHR. Thus, simultaneous determination of ANPmRNA and ANP levels is a refined strategy of investigation for the biosynthesis, storage and secretion of ANP.

Original languageEnglish
Pages (from-to)239-245
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume148
Issue number1
DOIs
Publication statusPublished - 1987 Oct 14
Externally publishedYes

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Inbred SHR Rats
Heart Atria
Rats
Atrial Natriuretic Factor
Messenger RNA
Peptides
Inbred WKY Rats
Biosynthesis
Tissue
Wistar Rats
Plasmas

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Simultaneous measurement of atrial natriuretic polypeptide (ANP) messenger RNA and ANP in rat heart - Evidence for a preferentially increased synthesis and secretion of ANP in left atrium of spontaneously hypertensive rats (SHR) -. / Arai, Hiroshi; Nakao, Kazuwa; Saito, Yoshihiko; Morii, Narito; Sugawara, Akira; Yamada, Takayuki; Itoh, Hiroshi; Shiono, Shozo; Mukoyama, Masashi; Ohkubo, Hiroaki; Nakanishi, Shigetada; Imura, Hiroo.

In: Biochemical and Biophysical Research Communications, Vol. 148, No. 1, 14.10.1987, p. 239-245.

Research output: Contribution to journalArticle

Arai, Hiroshi ; Nakao, Kazuwa ; Saito, Yoshihiko ; Morii, Narito ; Sugawara, Akira ; Yamada, Takayuki ; Itoh, Hiroshi ; Shiono, Shozo ; Mukoyama, Masashi ; Ohkubo, Hiroaki ; Nakanishi, Shigetada ; Imura, Hiroo. / Simultaneous measurement of atrial natriuretic polypeptide (ANP) messenger RNA and ANP in rat heart - Evidence for a preferentially increased synthesis and secretion of ANP in left atrium of spontaneously hypertensive rats (SHR) -. In: Biochemical and Biophysical Research Communications. 1987 ; Vol. 148, No. 1. pp. 239-245.
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abstract = "Tissue levels of atrial natriuretic polypeptide (ANP) messenger RNA (ANPmRNA) and ANP in the rat heart were measured simultaneously. In Wistar rats, ANPmRNA of the same size (approximately 0.95 kbp) was detected in all four chambers of the rat heart. The ANPmRNA level was the highest in the right atrium, and the left atrial level was slightly lower than the right atrial level. Ventricular levels were more than two orders of magnitude lower than atrial levels. Tissue ANP concentrations of four chambers were roughly parallel to ANPmRNA levels. In spontaneously hypertensive rats (SHR) with the elevated plasma ANP level, the ANPmRNA level in the left atrium was substantially increased. The left/right ratio of atrial ANPmRNA level in SHR (150{\%}) was higher than that in control Wistar Kyoto rats (WKY) (90{\%}). In contrast, the left/right ratio of atrial ANP concentration was decreased in SHR (44{\%}) compared with that in WKY (84{\%}). The ratio of ANP to ANPmRNA levels in the left atrium of SHR was about three times smaller than that in the right atrium of SHR, and those in bilateral atria of WKY. These results indicate that the biosynthesis and secretion of ANP from the left atrium is preferentially increased in SHR. Thus, simultaneous determination of ANPmRNA and ANP levels is a refined strategy of investigation for the biosynthesis, storage and secretion of ANP.",
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AU - Arai, Hiroshi

AU - Nakao, Kazuwa

AU - Saito, Yoshihiko

AU - Morii, Narito

AU - Sugawara, Akira

AU - Yamada, Takayuki

AU - Itoh, Hiroshi

AU - Shiono, Shozo

AU - Mukoyama, Masashi

AU - Ohkubo, Hiroaki

AU - Nakanishi, Shigetada

AU - Imura, Hiroo

PY - 1987/10/14

Y1 - 1987/10/14

N2 - Tissue levels of atrial natriuretic polypeptide (ANP) messenger RNA (ANPmRNA) and ANP in the rat heart were measured simultaneously. In Wistar rats, ANPmRNA of the same size (approximately 0.95 kbp) was detected in all four chambers of the rat heart. The ANPmRNA level was the highest in the right atrium, and the left atrial level was slightly lower than the right atrial level. Ventricular levels were more than two orders of magnitude lower than atrial levels. Tissue ANP concentrations of four chambers were roughly parallel to ANPmRNA levels. In spontaneously hypertensive rats (SHR) with the elevated plasma ANP level, the ANPmRNA level in the left atrium was substantially increased. The left/right ratio of atrial ANPmRNA level in SHR (150%) was higher than that in control Wistar Kyoto rats (WKY) (90%). In contrast, the left/right ratio of atrial ANP concentration was decreased in SHR (44%) compared with that in WKY (84%). The ratio of ANP to ANPmRNA levels in the left atrium of SHR was about three times smaller than that in the right atrium of SHR, and those in bilateral atria of WKY. These results indicate that the biosynthesis and secretion of ANP from the left atrium is preferentially increased in SHR. Thus, simultaneous determination of ANPmRNA and ANP levels is a refined strategy of investigation for the biosynthesis, storage and secretion of ANP.

AB - Tissue levels of atrial natriuretic polypeptide (ANP) messenger RNA (ANPmRNA) and ANP in the rat heart were measured simultaneously. In Wistar rats, ANPmRNA of the same size (approximately 0.95 kbp) was detected in all four chambers of the rat heart. The ANPmRNA level was the highest in the right atrium, and the left atrial level was slightly lower than the right atrial level. Ventricular levels were more than two orders of magnitude lower than atrial levels. Tissue ANP concentrations of four chambers were roughly parallel to ANPmRNA levels. In spontaneously hypertensive rats (SHR) with the elevated plasma ANP level, the ANPmRNA level in the left atrium was substantially increased. The left/right ratio of atrial ANPmRNA level in SHR (150%) was higher than that in control Wistar Kyoto rats (WKY) (90%). In contrast, the left/right ratio of atrial ANP concentration was decreased in SHR (44%) compared with that in WKY (84%). The ratio of ANP to ANPmRNA levels in the left atrium of SHR was about three times smaller than that in the right atrium of SHR, and those in bilateral atria of WKY. These results indicate that the biosynthesis and secretion of ANP from the left atrium is preferentially increased in SHR. Thus, simultaneous determination of ANPmRNA and ANP levels is a refined strategy of investigation for the biosynthesis, storage and secretion of ANP.

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