Single-cell bioluminescence imaging of deep tissue in freely moving animals

Satoshi Iwano, Mayu Sugiyama, Hiroshi Hama, Akiya Watakabe, Naomi Hasegawa, Takahiro Kuchimaru, Kazumasa Z. Tanaka, Megumu Takahashi, Yoko Ishida, Junichi Hata, Satoshi Shimozono, Kana Namiki, Takashi Fukano, Masahiro Kiyama, Hideyuki Okano, Shinae Kizaka-Kondoh, Thomas J. McHugh, Tetsuo Yamamori, Hiroyuki Hioki, Shojiro Maki & 1 others Atsushi Miyawaki

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Bioluminescence is a natural light source based on luciferase catalysis of its substrate luciferin. We performed directed evolution on firefly luciferase using a red-shifted and highly deliverable luciferin analog to establish AkaBLI, an all-engineered bioluminescence in vivo imaging system. AkaBLI produced emissions in vivo that were brighter by a factor of 100 to 1000 than conventional systems, allowing noninvasive visualization of single cells deep inside freely moving animals. Single tumorigenic cells trapped in the mouse lung vasculature could be visualized. In the mouse brain, genetic labeling with neural activity sensors allowed tracking of small clusters of hippocampal neurons activated by novel environments. In a marmoset, we recorded video-rate bioluminescence from neurons in the striatum, a deep brain area, for more than 1 year. AkaBLI is therefore a bioengineered light source to spur unprecedented scientific, medical, and industrial applications.

Original languageEnglish
Pages (from-to)935-939
Number of pages5
JournalScience
Volume359
Issue number6378
DOIs
Publication statusPublished - 2018 Feb 23
Externally publishedYes

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Firefly Luciferases
Neurons
Light
Callithrix
Brain
Luciferases
Catalysis
Lung

ASJC Scopus subject areas

  • General

Cite this

Iwano, S., Sugiyama, M., Hama, H., Watakabe, A., Hasegawa, N., Kuchimaru, T., ... Miyawaki, A. (2018). Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science, 359(6378), 935-939. https://doi.org/10.1126/science.aaq1067

Single-cell bioluminescence imaging of deep tissue in freely moving animals. / Iwano, Satoshi; Sugiyama, Mayu; Hama, Hiroshi; Watakabe, Akiya; Hasegawa, Naomi; Kuchimaru, Takahiro; Tanaka, Kazumasa Z.; Takahashi, Megumu; Ishida, Yoko; Hata, Junichi; Shimozono, Satoshi; Namiki, Kana; Fukano, Takashi; Kiyama, Masahiro; Okano, Hideyuki; Kizaka-Kondoh, Shinae; McHugh, Thomas J.; Yamamori, Tetsuo; Hioki, Hiroyuki; Maki, Shojiro; Miyawaki, Atsushi.

In: Science, Vol. 359, No. 6378, 23.02.2018, p. 935-939.

Research output: Contribution to journalArticle

Iwano, S, Sugiyama, M, Hama, H, Watakabe, A, Hasegawa, N, Kuchimaru, T, Tanaka, KZ, Takahashi, M, Ishida, Y, Hata, J, Shimozono, S, Namiki, K, Fukano, T, Kiyama, M, Okano, H, Kizaka-Kondoh, S, McHugh, TJ, Yamamori, T, Hioki, H, Maki, S & Miyawaki, A 2018, 'Single-cell bioluminescence imaging of deep tissue in freely moving animals', Science, vol. 359, no. 6378, pp. 935-939. https://doi.org/10.1126/science.aaq1067
Iwano S, Sugiyama M, Hama H, Watakabe A, Hasegawa N, Kuchimaru T et al. Single-cell bioluminescence imaging of deep tissue in freely moving animals. Science. 2018 Feb 23;359(6378):935-939. https://doi.org/10.1126/science.aaq1067
Iwano, Satoshi ; Sugiyama, Mayu ; Hama, Hiroshi ; Watakabe, Akiya ; Hasegawa, Naomi ; Kuchimaru, Takahiro ; Tanaka, Kazumasa Z. ; Takahashi, Megumu ; Ishida, Yoko ; Hata, Junichi ; Shimozono, Satoshi ; Namiki, Kana ; Fukano, Takashi ; Kiyama, Masahiro ; Okano, Hideyuki ; Kizaka-Kondoh, Shinae ; McHugh, Thomas J. ; Yamamori, Tetsuo ; Hioki, Hiroyuki ; Maki, Shojiro ; Miyawaki, Atsushi. / Single-cell bioluminescence imaging of deep tissue in freely moving animals. In: Science. 2018 ; Vol. 359, No. 6378. pp. 935-939.
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