Single-cell-derived mesenchymal stem cells overexpressing Csx/Nkx2.5 and GATA4 undergo the stochastic cardiomyogenic fate and behave like transient amplifying cells

Yoji Yamada, Kazuhiro Sakurada, Yukiji Takeda, Satoshi Gojo, Akihiro Umezawa

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

Bone marrow-derived stromal cells can give rise to cardiomyocytes as well as adipocytes, osteocytes, and chondrocytes in vitro. The existence of mesenchymal stem cells has been proposed, but it remains unclear if a single-cell-derived stem cell stochastically commits toward a cardiac lineage. By single-cell marking, we performed a follow-up study of individual cells during the differentiation of 9-15c mesenchymal stromal cells derived from bone marrow cells. Three types of cells, i.e., cardiac myoblasts, cardiac progenitors and multipotent stem cells were differentiated from a single cell, implying that cardiomyocytes are generated stochastically from a single-cell-derived stem cell. We also demonstrated that overexpression of Csx/Nkx2.5 and GATA4, precardiac mesodermal transcription factors, enhanced cardiomyogenic differentiation of 9-15c cells, and the frequency of cardiomyogenic differentiation was increased by co-culturing with fetal cardiomyocytes. Single-cell-derived mesenchymal stem cells overexpressing Csx/Nkx2.5 and GATA4 behaved like cardiac transient amplifying cells, and still retained their plasticity in vivo.

Original languageEnglish
Pages (from-to)698-706
Number of pages9
JournalExperimental Cell Research
Volume313
Issue number4
DOIs
Publication statusPublished - 2007 Feb 15
Externally publishedYes

Keywords

  • Cardiomyocytes
  • Csx/Nkx2.5
  • GATA4
  • Mesenchymal stem cells
  • Transient amplifying cells

ASJC Scopus subject areas

  • Cell Biology

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