Single-cell sequencing of neonatal uterus reveals an Misr2+ endometrial progenitor indispensable for fertility

Hatice Duygu Saatcioglu, Motohiro Kano, Heiko Horn, Lihua Zhang, Wesley Samore, Nicholas Nagykery, Marie Charlotte Meinsohn, Minsuk Hyun, Rana Suliman, Joy Poulo, Jennifer Hsu, Caitlin Sacha, Dan Wang, Guangping Gao, Kasper Lage, Esther Oliva, Mary E. Morris Sabatini, Patricia K. Donahoe, David Pépin

Research output: Contribution to journalArticle

Abstract

The Mullerian ducts are the anlagen of the female reproductive tract, which regress in the male fetus in response to MIS. This process is driven by subluminal mesenchymal cells expressing Misr2, which trigger the regression of the adjacent Mullerian ductal epithelium. In females, these Misr2+ cells are retained, yet their contribution to the development of the uterus remains unknown. Here, we report that subluminal Misr2+ cells persist postnatally in the uterus of rodents, but recede by week 37 of gestation in humans. Using single-cell RNA sequencing, we demonstrate that ectopic postnatal MIS administration inhibits these cells and prevents the formation of endometrial stroma in rodents, suggesting a progenitor function. Exposure to MIS during the first six days of life, by inhibiting specification of the stroma, dysregulates paracrine signals necessary for uterine development, eventually resulting in apoptosis of the Misr2+ cells, uterine hypoplasia, and complete infertility in the adult female.

Original languageEnglish
JournaleLife
Volume8
DOIs
Publication statusPublished - 2019 Jun 24
Externally publishedYes

Fingerprint

Management information systems
Uterus
Fertility
Ducts
Rodentia
Cells
RNA
Mullerian Ducts
Apoptosis
RNA Sequence Analysis
Specifications
Infertility
Fetus
Epithelium
Pregnancy

Keywords

  • AMH
  • developmental biology
  • human
  • infertility
  • mesenchyme
  • MIS
  • mouse
  • Mullerian duct
  • rat
  • uterus development

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Single-cell sequencing of neonatal uterus reveals an Misr2+ endometrial progenitor indispensable for fertility. / Saatcioglu, Hatice Duygu; Kano, Motohiro; Horn, Heiko; Zhang, Lihua; Samore, Wesley; Nagykery, Nicholas; Meinsohn, Marie Charlotte; Hyun, Minsuk; Suliman, Rana; Poulo, Joy; Hsu, Jennifer; Sacha, Caitlin; Wang, Dan; Gao, Guangping; Lage, Kasper; Oliva, Esther; Morris Sabatini, Mary E.; Donahoe, Patricia K.; Pépin, David.

In: eLife, Vol. 8, 24.06.2019.

Research output: Contribution to journalArticle

Saatcioglu, HD, Kano, M, Horn, H, Zhang, L, Samore, W, Nagykery, N, Meinsohn, MC, Hyun, M, Suliman, R, Poulo, J, Hsu, J, Sacha, C, Wang, D, Gao, G, Lage, K, Oliva, E, Morris Sabatini, ME, Donahoe, PK & Pépin, D 2019, 'Single-cell sequencing of neonatal uterus reveals an Misr2+ endometrial progenitor indispensable for fertility', eLife, vol. 8. https://doi.org/10.7554/eLife.46349
Saatcioglu, Hatice Duygu ; Kano, Motohiro ; Horn, Heiko ; Zhang, Lihua ; Samore, Wesley ; Nagykery, Nicholas ; Meinsohn, Marie Charlotte ; Hyun, Minsuk ; Suliman, Rana ; Poulo, Joy ; Hsu, Jennifer ; Sacha, Caitlin ; Wang, Dan ; Gao, Guangping ; Lage, Kasper ; Oliva, Esther ; Morris Sabatini, Mary E. ; Donahoe, Patricia K. ; Pépin, David. / Single-cell sequencing of neonatal uterus reveals an Misr2+ endometrial progenitor indispensable for fertility. In: eLife. 2019 ; Vol. 8.
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AU - Gao, Guangping

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