Single-cell study of neural stem cells derived from human iPSCs reveals distinct progenitor populations with neurogenic and gliogenic potential

Matti Lam, Tsukasa Sanosaka, Anders Lundin, Kent Imaizumi, Damla Etal, Fredrik H. Karlsson, Maryam Clausen, Jonathan Cairns, Ryan Hicks, Jun Kohyama, Malin Kele, Hideyuki Okano, Anna Falk

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

We used single-cell RNA sequencing (seq) on several human induced pluripotent stem (iPS) cell-derived neural stem cell (NSC) lines and one fetal brain-derived NSC line to study inherent cell type heterogeneity at proliferating neural stem cell stage and uncovered predisposed presence of neurogenic and gliogenic progenitors. We observed heterogeneity in neurogenic progenitors that differed between the iPS cell-derived NSC lines and the fetal-derived NSC line, and we also observed differences in spontaneous differentiation potential for inhibitory and excitatory neurons between the iPS cell-derived NSC lines and the fetal-derived NSC line. In addition, using a recently published glia patterning protocol we enriched for gliogenic progenitors and generated glial cells from an iPS cell-derived NSC line.

Original languageEnglish
Pages (from-to)836-847
Number of pages12
JournalGenes to Cells
Volume24
Issue number12
DOIs
Publication statusPublished - 2019 Dec 1

Keywords

  • glia
  • induced pluripotent stem cells
  • modeling gliogenesis
  • modeling neurogenesis
  • neural stem cells
  • neurons
  • single cell RNA sequencing

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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