Single-cell suspension assay with an MTT end point is useful for evaluating the optimal adjuvant chemotherapy for advanced gastric cancer

Yoshiro Saikawa, Tetsuro Kubota, Toshiharu Furukawa, Akihiko Suto, Masahiko Watanabe, Koichiro Kumai, Kyuya Ishibiki, Masaki Kitajima

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

One hundred and forty-eight patients with gastric cancer admitted to Keio University Hospital between July 1988 and October 1992 underwent resection of the primary lesion, as well as single-cell suspension assay of fresh surgical materials with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT assay) for chemosensitivity evaluation. Fifty patients with histologically stage III or IV gastric cancer were enrolled in this study, among whom 10 received no chemotherapy after surgery while 40 received chemotherapy at equivalent dose levels after surgery. The patients given chemotherapy were divided into two groups consisting of an "Adapted" group treated with at least one agent identified as effective by the assay, and a "Non-adapted" group treated with agents to which the cells were not sensitive in the assay, in order to identify the optimal cut-off inhibition rate (IR) in MTT assay for evaluation of the appropriate adjuvant cancer chemotherapy after surgery. A cut-off IR of 30% was optimal for differentiating the survival rates between the "Adapted" and "Non-adapted" groups. Patients treated with drugs which showed more than 30% IR on their surgical specimens showed a better survival rate than patients treated with drugs which were ineffective in the assay.

Original languageEnglish
Pages (from-to)762-765
Number of pages4
JournalJapanese Journal of Cancer Research
Volume85
Issue number7
Publication statusPublished - 1994 Jul

Fingerprint

Adjuvant Chemotherapy
Stomach Neoplasms
Suspensions
Drug Therapy
Survival Rate
Pharmaceutical Preparations
Neoplasms

Keywords

  • Chemosensitivity test
  • Gastric cancer
  • MTT assay

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Single-cell suspension assay with an MTT end point is useful for evaluating the optimal adjuvant chemotherapy for advanced gastric cancer. / Saikawa, Yoshiro; Kubota, Tetsuro; Furukawa, Toshiharu; Suto, Akihiko; Watanabe, Masahiko; Kumai, Koichiro; Ishibiki, Kyuya; Kitajima, Masaki.

In: Japanese Journal of Cancer Research, Vol. 85, No. 7, 07.1994, p. 762-765.

Research output: Contribution to journalArticle

Saikawa, Yoshiro ; Kubota, Tetsuro ; Furukawa, Toshiharu ; Suto, Akihiko ; Watanabe, Masahiko ; Kumai, Koichiro ; Ishibiki, Kyuya ; Kitajima, Masaki. / Single-cell suspension assay with an MTT end point is useful for evaluating the optimal adjuvant chemotherapy for advanced gastric cancer. In: Japanese Journal of Cancer Research. 1994 ; Vol. 85, No. 7. pp. 762-765.
@article{2e18a66a74124078b453ceb395a83f95,
title = "Single-cell suspension assay with an MTT end point is useful for evaluating the optimal adjuvant chemotherapy for advanced gastric cancer",
abstract = "One hundred and forty-eight patients with gastric cancer admitted to Keio University Hospital between July 1988 and October 1992 underwent resection of the primary lesion, as well as single-cell suspension assay of fresh surgical materials with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT assay) for chemosensitivity evaluation. Fifty patients with histologically stage III or IV gastric cancer were enrolled in this study, among whom 10 received no chemotherapy after surgery while 40 received chemotherapy at equivalent dose levels after surgery. The patients given chemotherapy were divided into two groups consisting of an {"}Adapted{"} group treated with at least one agent identified as effective by the assay, and a {"}Non-adapted{"} group treated with agents to which the cells were not sensitive in the assay, in order to identify the optimal cut-off inhibition rate (IR) in MTT assay for evaluation of the appropriate adjuvant cancer chemotherapy after surgery. A cut-off IR of 30{\%} was optimal for differentiating the survival rates between the {"}Adapted{"} and {"}Non-adapted{"} groups. Patients treated with drugs which showed more than 30{\%} IR on their surgical specimens showed a better survival rate than patients treated with drugs which were ineffective in the assay.",
keywords = "Chemosensitivity test, Gastric cancer, MTT assay",
author = "Yoshiro Saikawa and Tetsuro Kubota and Toshiharu Furukawa and Akihiko Suto and Masahiko Watanabe and Koichiro Kumai and Kyuya Ishibiki and Masaki Kitajima",
year = "1994",
month = "7",
language = "English",
volume = "85",
pages = "762--765",
journal = "Cancer Science",
issn = "1347-9032",
publisher = "Wiley-Blackwell",
number = "7",

}

TY - JOUR

T1 - Single-cell suspension assay with an MTT end point is useful for evaluating the optimal adjuvant chemotherapy for advanced gastric cancer

AU - Saikawa, Yoshiro

AU - Kubota, Tetsuro

AU - Furukawa, Toshiharu

AU - Suto, Akihiko

AU - Watanabe, Masahiko

AU - Kumai, Koichiro

AU - Ishibiki, Kyuya

AU - Kitajima, Masaki

PY - 1994/7

Y1 - 1994/7

N2 - One hundred and forty-eight patients with gastric cancer admitted to Keio University Hospital between July 1988 and October 1992 underwent resection of the primary lesion, as well as single-cell suspension assay of fresh surgical materials with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT assay) for chemosensitivity evaluation. Fifty patients with histologically stage III or IV gastric cancer were enrolled in this study, among whom 10 received no chemotherapy after surgery while 40 received chemotherapy at equivalent dose levels after surgery. The patients given chemotherapy were divided into two groups consisting of an "Adapted" group treated with at least one agent identified as effective by the assay, and a "Non-adapted" group treated with agents to which the cells were not sensitive in the assay, in order to identify the optimal cut-off inhibition rate (IR) in MTT assay for evaluation of the appropriate adjuvant cancer chemotherapy after surgery. A cut-off IR of 30% was optimal for differentiating the survival rates between the "Adapted" and "Non-adapted" groups. Patients treated with drugs which showed more than 30% IR on their surgical specimens showed a better survival rate than patients treated with drugs which were ineffective in the assay.

AB - One hundred and forty-eight patients with gastric cancer admitted to Keio University Hospital between July 1988 and October 1992 underwent resection of the primary lesion, as well as single-cell suspension assay of fresh surgical materials with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT assay) for chemosensitivity evaluation. Fifty patients with histologically stage III or IV gastric cancer were enrolled in this study, among whom 10 received no chemotherapy after surgery while 40 received chemotherapy at equivalent dose levels after surgery. The patients given chemotherapy were divided into two groups consisting of an "Adapted" group treated with at least one agent identified as effective by the assay, and a "Non-adapted" group treated with agents to which the cells were not sensitive in the assay, in order to identify the optimal cut-off inhibition rate (IR) in MTT assay for evaluation of the appropriate adjuvant cancer chemotherapy after surgery. A cut-off IR of 30% was optimal for differentiating the survival rates between the "Adapted" and "Non-adapted" groups. Patients treated with drugs which showed more than 30% IR on their surgical specimens showed a better survival rate than patients treated with drugs which were ineffective in the assay.

KW - Chemosensitivity test

KW - Gastric cancer

KW - MTT assay

UR - http://www.scopus.com/inward/record.url?scp=0028306253&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028306253&partnerID=8YFLogxK

M3 - Article

C2 - 8071118

AN - SCOPUS:0028306253

VL - 85

SP - 762

EP - 765

JO - Cancer Science

JF - Cancer Science

SN - 1347-9032

IS - 7

ER -