Single molecular multianalyte (Ca2+, Mg2+) fluorescent probe and applications to bioimaging

Hirokazu Komatsu, Takahiro Miki, Daniel Citterio, Takeshi Kubota, Yutaka Shindo, Yoshiichiro Kitamura, Kotaro Oka, Koji Suzuki

Research output: Contribution to journalArticle

197 Citations (Scopus)

Abstract

Intracellular signal transduction relies on spatial and temporal signal transmitter dynamics. To clarify the correlations of these transmitter molecules, multicolor-imaging has been widely used. However, in the case of applying multiple indicators in a cell, spectral overlap of the indicators prevents accurate quantitative analysis. Moreover, the invasive (toxic) effect, the localization, the metabolism, as well as photobleaching of these indicators complicate the situation. Here, we show that single-molecular multifluorescent probes can overcome these problems. While intracellular calcium plays a critical role as a signal transmitter and magnesium acts as a cofactor in many situations, the correlations between the two cations are now the main issue. We designed and synthesized a Ca2+-Mg2+ responsive multifluorescent probe, KCM-1. KCM-1 shows a spectral blue shift upon complexation to Ca2+ and a red shift to the presence of Mg2+. With data analyzed at different excitation wavelengths, the concentrations of Ca2+ and Mg2+ are simultaneously quantified. Furthermore, by using the AM-ester method, intracellular Ca2+ and Mg2+ concentrations are simultaneously imaged. Such a type of intracellular multiple analyte imaging by a single-molecular multifluorescent probe is successfully demonstrated for the first time.

Original languageEnglish
Pages (from-to)10798-10799
Number of pages2
JournalJournal of the American Chemical Society
Volume127
Issue number31
DOIs
Publication statusPublished - 2005 Aug 10

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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