Single molecule energetics of F1-ATPase motor

Eiro Muneyuki, Takahiro Watanabe-Nakayama, Tetsuya Suzuki, Masasuke Yoshida, Takayuki Nishizaka, Hiroyuki Noji

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Abstract

Motor proteins are essential in life processes because they convert the free energy of ATP hydrolysis to mechanical work. However, the fundamental question on how they work when different amounts of free energy are released after ATP hydrolysis remains unanswered. To answer this question, it is essential to clarify how the stepping motion of a motor protein reflects the concentrations of ATP, ADP, and Pi in its individual actions at a single molecule level. The F1 portion of ATP synthase, also called F 1-ATPase, is a rotary molecular motor in which the central γ-subunit rotates against the α3b3 cylinder. The motor exhibits clear step motion at low ATP concentrations. The rotary action of this motor is processive and generates a high torque. These features are ideal for exploring the relationship between free energy input and mechanical work output, but there is a serious problem in that this motor is severely inhibited by ADP. In this study, we overcame this problem of ADP inhibition by introducing several mutations while retaining high enzymatic activity. Using a probe of attached beads, stepping rotation against viscous load was examined at a wide range of free energy values by changing the ADP concentration. The results showed that the apparent work of each individual step motion was not affected by the free energy of ATP hydrolysis, but the frequency of each individual step motion depended on the free energy. This is the first study that examined the stepping motion of a molecular motor at a single molecule level with simultaneous systematic control of ΔGATP. The results imply that microscopically defined work at a single molecule level cannot be directly compared with macroscopically defined free energy input.

Original languageEnglish
Pages (from-to)1806-1812
Number of pages7
JournalBiophysical Journal
Volume92
Issue number5
DOIs
Publication statusPublished - 2007 Mar
Externally publishedYes

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ASJC Scopus subject areas

  • Biophysics

Cite this

Muneyuki, E., Watanabe-Nakayama, T., Suzuki, T., Yoshida, M., Nishizaka, T., & Noji, H. (2007). Single molecule energetics of F1-ATPase motor. Biophysical Journal, 92(5), 1806-1812. https://doi.org/10.1529/biophysj.106.097170