Single nanobeads detection by scanning laser spot for immunosensing

Koji Tsujita, Yuichi Hasegawa, Masayuki Ono, Makoto Itonaga, Yasuaki Kabe, Satoshi Sakamoto, Hiroshi Handa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We devised a new measurement system for a small number of biomolecules by using nanobeads and optical disc technologies. The antibodies were specifically immobilized onto optical disc surface, then the biomolecules were captured with antibody-modified nanobeads by immunoreaction. Each nanobead was detected one by one with a small scanning laser spot generated by an optical pickup for Blu-ray Disc. The number of the nanobeads corresponds to the number of the biomolecules, therefore the biomolecules can be measured by counting the nanobeads. This paper demonstrates the quantitative detection of a biomolecule by our method.

Original languageEnglish
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages2297-2299
Number of pages3
ISBN (Electronic)9780979806476
Publication statusPublished - 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: 2014 Oct 262014 Oct 30

Publication series

Name18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period14/10/2614/10/30

Keywords

  • Biomolecule
  • Digital counting
  • Immunoassay
  • Nanobeads
  • Optical disc

ASJC Scopus subject areas

  • Control and Systems Engineering

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