Single neuron observation in a 3D neuronal tissue blook

Midori Kato-Negishi, Hiroaki Onoe, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper describes a method to observe a single neuron in a three-dimensional (3D) neuronal tissue formed by bottom-up tissue construction. We first formed GFP-actin-transfected neurospheroids for visualization of neurons, and then formed a millimeter-sized neuronal block using PDMS mold chambers. We found that GFP-actin-transfected neurons in the neuronal block have in vivo-like morphology and 3D extension of their axons/dendrites compared to 2D culture system, and that many spines were successfully formed on the dendrites. We are confident that our neuronal block enables morphological maturation of the neurons due to its in vivo-like 3D environment, and that our bottom-up construction to observe single neurons is a powerful approach for various fields including analysis of single neuronal behaviors in vivo, and pharmacological assay for drug screening.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1144-1146
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012 Jan 1
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 2012 Oct 282012 Nov 1

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12/10/2812/11/1

Keywords

  • 3D tissue engineering
  • Cortex
  • GFP-Actin
  • Neuron
  • Transfection

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Kato-Negishi, M., Onoe, H., & Takeuchi, S. (2012). Single neuron observation in a 3D neuronal tissue blook. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1144-1146). (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012). Chemical and Biological Microsystems Society.