Single nucleotide polymorphisms of the inflammatory cytokine genes in adults with chronic immune thrombocytopenic purpura

Takashi Satoh, Janardan P. Pandey, Yuka Okazaki, Hidekata Yasuoka, Yutaka Kawakami, Yasuo Ikeda, Masataka Kuwana

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Single nucleotide polymorphisms (SNPs) of inflammatory cytokine genes were examined in 84 adult Japanese patients with chronic immune thrombocytopenic purpura (ITP) and 56 race-matched healthy controls. The SNPs examined were within the genes encoding tumour necrosis factor (TNF)-α (-238 G/A and -308 G/A), TNF-β (+252 G/A), and interleukin (IL)-1β (-511 C/T and +3953 T/C). Of these SNPs, the frequency of the TNF-β (+252) G/G phenotype was significantly higher in ITP patients than in healthy controls (21% vs. 7%, P = 0.04, odds ratio = 3.6, 95% confidence interval 1.1-11.1), while no significant association was detected for the other SNPs. The distribution of the TNF-β (+252) phenotype was not associated with human leucocyte antigen class II alleles or the therapeutic response in ITP patients. The frequency of circulating anti-glycoprotein IIb/IIIa antibody-producing B cells was significantly higher in ITP patients with the TNF-β (+252) G/G phenotype than in those with the G/A or A/A phenotype (11.9 ± 4.9 vs. 6.8 ± 4.9 and 3.7 ± 2.8 per 105 peripheral blood mononuclear cells; P = 0.02 and P < 0.001, respectively). These findings suggest that the SNP located at TNF-β (+252) contributes to susceptibility to chronic ITP by controlling the autoreactive B-cell responses to platelet membrane glycoproteins.

Original languageEnglish
Pages (from-to)796-801
Number of pages6
JournalBritish Journal of Haematology
Volume124
Issue number6
DOIs
Publication statusPublished - 2004 Mar 1

Keywords

  • Anti-platelet autoantibody
  • Immune thrombocytopenic purpura
  • Inflammatory cytokine
  • Single nucleotide polymorphism
  • Tumour necrosis factor-β

ASJC Scopus subject areas

  • Hematology

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