Single-step bioluminescence lateral flow immunoassays for diagnostics

Riho Shimazu, Junnosuke Kawahara, Kosuke Tomimuro, Kazushi Misawa, Yan Ni, Yuki Hiruta, Maarten Merkx, Daniel Citterio

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present two diagnostic platforms, a microfluidic thread-based and a conventional immunochromatographic strip approach, for single-step lateral flow immunoassays (LFIAs) with bioluminescence (BL) signaling requiring only sample application and detection of emission color changes by a digital camera. Both rely on bioluminescence resonance energy transfer (BRET)-switching proteins. The detection of three clinically relevant targets is demonstrated: Cetuximab as example of an antibody-based anticancer drug requiring monitoring of blood drug levels in patients, and HIV1-p17 antibodies and antigens as examples of infectious disease markers.

Original languageEnglish
Title of host publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PublisherChemical and Biological Microsystems Society
Pages703-704
Number of pages2
ISBN (Electronic)9781733419000
Publication statusPublished - 2019
Event23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland
Duration: 2019 Oct 272019 Oct 31

Publication series

Name23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Conference

Conference23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
CountrySwitzerland
CityBasel
Period19/10/2719/10/31

Keywords

  • Bioluminescence
  • Blood analysis
  • BRET
  • Therapeutic drug monitoring
  • Thread-based devices

ASJC Scopus subject areas

  • Bioengineering
  • Chemical Engineering (miscellaneous)

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