TY - GEN
T1 - Single-step bioluminescence lateral flow immunoassays for diagnostics
AU - Shimazu, Riho
AU - Kawahara, Junnosuke
AU - Tomimuro, Kosuke
AU - Misawa, Kazushi
AU - Ni, Yan
AU - Hiruta, Yuki
AU - Merkx, Maarten
AU - Citterio, Daniel
N1 - Funding Information:
This work has been supported by a JSPS Bilateral Joint Research Project grant.
Publisher Copyright:
© 2019 CBMS-0001.
PY - 2019
Y1 - 2019
N2 - We present two diagnostic platforms, a microfluidic thread-based and a conventional immunochromatographic strip approach, for single-step lateral flow immunoassays (LFIAs) with bioluminescence (BL) signaling requiring only sample application and detection of emission color changes by a digital camera. Both rely on bioluminescence resonance energy transfer (BRET)-switching proteins. The detection of three clinically relevant targets is demonstrated: Cetuximab as example of an antibody-based anticancer drug requiring monitoring of blood drug levels in patients, and HIV1-p17 antibodies and antigens as examples of infectious disease markers.
AB - We present two diagnostic platforms, a microfluidic thread-based and a conventional immunochromatographic strip approach, for single-step lateral flow immunoassays (LFIAs) with bioluminescence (BL) signaling requiring only sample application and detection of emission color changes by a digital camera. Both rely on bioluminescence resonance energy transfer (BRET)-switching proteins. The detection of three clinically relevant targets is demonstrated: Cetuximab as example of an antibody-based anticancer drug requiring monitoring of blood drug levels in patients, and HIV1-p17 antibodies and antigens as examples of infectious disease markers.
KW - BRET
KW - Bioluminescence
KW - Blood analysis
KW - Therapeutic drug monitoring
KW - Thread-based devices
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M3 - Conference contribution
AN - SCOPUS:85094966640
T3 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
SP - 703
EP - 704
BT - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PB - Chemical and Biological Microsystems Society
T2 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Y2 - 27 October 2019 through 31 October 2019
ER -
