Sirt1 plays an important role in mediating greater functionality of human ES/iPS-derived vascular endothelial cells

Koichiro Honma, Masakatsu Sone, Daisuke Taura, Kenichi Yamahara, Yutaka Suzuki, Kazutoshi Takahashi, Takuhiro Sonoyama, Megumi Inuzuka, Yasutomo Fukunaga, Naohisa Tamura, Hiroshi Itoh, Shinya Yamanaka, Kazuwa Nakao

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Objective: We previously succeeded in inducing and isolating vascular endothelial cells (ECs) from both human embryonic stem (ES) and induced pluripotent stem (iPS) cells. Here, we compared the functionality of human adult ECs (HAECs), human ES-derived ECs (ESECs) and human iPS-derived ECs (iPSECs). Methods and results: We compared the cell proliferative potential, potential for migration, and tolerance to oxidative stress. ESECs were significantly superior to HAECs in all of these cell functions. The cell functions of iPSECs were comparable to those of ESECSs and also superior to HAECs. We then analyzed the gene expressions of HAECs, ESECs and iPSECs, and observed that the expression level of Sirt1, a nicotinamide adenine dinucleotide (NAD+)-dependent histone deacetylase, is higher in ESECs and iPSECs than in HAECs. The inhibition of Sirt1 with a Sirt1-specific inhibitor and siRNA antagonized these differences between the three types of cells. Conclusions: Sirt1 plays a key role in the high cellular function of ESECs and iPSECs. Although further in vivo investigations are required, this study initially demonstrated the potential of ESECs and iPSECs as the cell source for regenerative medicine, and also showed the potential of ES cells as a useful tool for elucidating the molecular mechanism of cell aging.

Original languageEnglish
Pages (from-to)42-47
Number of pages6
JournalAtherosclerosis
Volume212
Issue number1
DOIs
Publication statusPublished - 2010 Sep

Fingerprint

Endothelial Cells
NAD
Induced Pluripotent Stem Cells
Regenerative Medicine
Histone Deacetylases
Cell Aging
Embryonic Stem Cells
Small Interfering RNA
Oxidative Stress
Gene Expression

Keywords

  • Endothelium
  • Sirt1
  • Stem cells

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Sirt1 plays an important role in mediating greater functionality of human ES/iPS-derived vascular endothelial cells. / Honma, Koichiro; Sone, Masakatsu; Taura, Daisuke; Yamahara, Kenichi; Suzuki, Yutaka; Takahashi, Kazutoshi; Sonoyama, Takuhiro; Inuzuka, Megumi; Fukunaga, Yasutomo; Tamura, Naohisa; Itoh, Hiroshi; Yamanaka, Shinya; Nakao, Kazuwa.

In: Atherosclerosis, Vol. 212, No. 1, 09.2010, p. 42-47.

Research output: Contribution to journalArticle

Honma, K, Sone, M, Taura, D, Yamahara, K, Suzuki, Y, Takahashi, K, Sonoyama, T, Inuzuka, M, Fukunaga, Y, Tamura, N, Itoh, H, Yamanaka, S & Nakao, K 2010, 'Sirt1 plays an important role in mediating greater functionality of human ES/iPS-derived vascular endothelial cells', Atherosclerosis, vol. 212, no. 1, pp. 42-47. https://doi.org/10.1016/j.atherosclerosis.2010.04.021
Honma, Koichiro ; Sone, Masakatsu ; Taura, Daisuke ; Yamahara, Kenichi ; Suzuki, Yutaka ; Takahashi, Kazutoshi ; Sonoyama, Takuhiro ; Inuzuka, Megumi ; Fukunaga, Yasutomo ; Tamura, Naohisa ; Itoh, Hiroshi ; Yamanaka, Shinya ; Nakao, Kazuwa. / Sirt1 plays an important role in mediating greater functionality of human ES/iPS-derived vascular endothelial cells. In: Atherosclerosis. 2010 ; Vol. 212, No. 1. pp. 42-47.
@article{4cb89d29c46b49ac85b2708b1df49d55,
title = "Sirt1 plays an important role in mediating greater functionality of human ES/iPS-derived vascular endothelial cells",
abstract = "Objective: We previously succeeded in inducing and isolating vascular endothelial cells (ECs) from both human embryonic stem (ES) and induced pluripotent stem (iPS) cells. Here, we compared the functionality of human adult ECs (HAECs), human ES-derived ECs (ESECs) and human iPS-derived ECs (iPSECs). Methods and results: We compared the cell proliferative potential, potential for migration, and tolerance to oxidative stress. ESECs were significantly superior to HAECs in all of these cell functions. The cell functions of iPSECs were comparable to those of ESECSs and also superior to HAECs. We then analyzed the gene expressions of HAECs, ESECs and iPSECs, and observed that the expression level of Sirt1, a nicotinamide adenine dinucleotide (NAD+)-dependent histone deacetylase, is higher in ESECs and iPSECs than in HAECs. The inhibition of Sirt1 with a Sirt1-specific inhibitor and siRNA antagonized these differences between the three types of cells. Conclusions: Sirt1 plays a key role in the high cellular function of ESECs and iPSECs. Although further in vivo investigations are required, this study initially demonstrated the potential of ESECs and iPSECs as the cell source for regenerative medicine, and also showed the potential of ES cells as a useful tool for elucidating the molecular mechanism of cell aging.",
keywords = "Endothelium, Sirt1, Stem cells",
author = "Koichiro Honma and Masakatsu Sone and Daisuke Taura and Kenichi Yamahara and Yutaka Suzuki and Kazutoshi Takahashi and Takuhiro Sonoyama and Megumi Inuzuka and Yasutomo Fukunaga and Naohisa Tamura and Hiroshi Itoh and Shinya Yamanaka and Kazuwa Nakao",
year = "2010",
month = "9",
doi = "10.1016/j.atherosclerosis.2010.04.021",
language = "English",
volume = "212",
pages = "42--47",
journal = "Atherosclerosis",
issn = "0021-9150",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Sirt1 plays an important role in mediating greater functionality of human ES/iPS-derived vascular endothelial cells

AU - Honma, Koichiro

AU - Sone, Masakatsu

AU - Taura, Daisuke

AU - Yamahara, Kenichi

AU - Suzuki, Yutaka

AU - Takahashi, Kazutoshi

AU - Sonoyama, Takuhiro

AU - Inuzuka, Megumi

AU - Fukunaga, Yasutomo

AU - Tamura, Naohisa

AU - Itoh, Hiroshi

AU - Yamanaka, Shinya

AU - Nakao, Kazuwa

PY - 2010/9

Y1 - 2010/9

N2 - Objective: We previously succeeded in inducing and isolating vascular endothelial cells (ECs) from both human embryonic stem (ES) and induced pluripotent stem (iPS) cells. Here, we compared the functionality of human adult ECs (HAECs), human ES-derived ECs (ESECs) and human iPS-derived ECs (iPSECs). Methods and results: We compared the cell proliferative potential, potential for migration, and tolerance to oxidative stress. ESECs were significantly superior to HAECs in all of these cell functions. The cell functions of iPSECs were comparable to those of ESECSs and also superior to HAECs. We then analyzed the gene expressions of HAECs, ESECs and iPSECs, and observed that the expression level of Sirt1, a nicotinamide adenine dinucleotide (NAD+)-dependent histone deacetylase, is higher in ESECs and iPSECs than in HAECs. The inhibition of Sirt1 with a Sirt1-specific inhibitor and siRNA antagonized these differences between the three types of cells. Conclusions: Sirt1 plays a key role in the high cellular function of ESECs and iPSECs. Although further in vivo investigations are required, this study initially demonstrated the potential of ESECs and iPSECs as the cell source for regenerative medicine, and also showed the potential of ES cells as a useful tool for elucidating the molecular mechanism of cell aging.

AB - Objective: We previously succeeded in inducing and isolating vascular endothelial cells (ECs) from both human embryonic stem (ES) and induced pluripotent stem (iPS) cells. Here, we compared the functionality of human adult ECs (HAECs), human ES-derived ECs (ESECs) and human iPS-derived ECs (iPSECs). Methods and results: We compared the cell proliferative potential, potential for migration, and tolerance to oxidative stress. ESECs were significantly superior to HAECs in all of these cell functions. The cell functions of iPSECs were comparable to those of ESECSs and also superior to HAECs. We then analyzed the gene expressions of HAECs, ESECs and iPSECs, and observed that the expression level of Sirt1, a nicotinamide adenine dinucleotide (NAD+)-dependent histone deacetylase, is higher in ESECs and iPSECs than in HAECs. The inhibition of Sirt1 with a Sirt1-specific inhibitor and siRNA antagonized these differences between the three types of cells. Conclusions: Sirt1 plays a key role in the high cellular function of ESECs and iPSECs. Although further in vivo investigations are required, this study initially demonstrated the potential of ESECs and iPSECs as the cell source for regenerative medicine, and also showed the potential of ES cells as a useful tool for elucidating the molecular mechanism of cell aging.

KW - Endothelium

KW - Sirt1

KW - Stem cells

UR - http://www.scopus.com/inward/record.url?scp=77956230709&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956230709&partnerID=8YFLogxK

U2 - 10.1016/j.atherosclerosis.2010.04.021

DO - 10.1016/j.atherosclerosis.2010.04.021

M3 - Article

C2 - 20488443

AN - SCOPUS:77956230709

VL - 212

SP - 42

EP - 47

JO - Atherosclerosis

JF - Atherosclerosis

SN - 0021-9150

IS - 1

ER -