Site-specific chemosensitivity of human small-cell lung carcinoma growing orthotopically compared to subcutaneously in SCID mice

The importance of orthotopic models to obtain relevant drug evaluation data

T. H. Kuo, T. Kubota, M. Watanabe, Toshiharu Furukawa, S. Kase, H. Tanino, Y. Saikawa, K. Ishibiki, M. Kitajima, R. M. Hoffman

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

We have developed a novel in vivo model of human small-cell lung carcinoma (SCLC) using orthotopic reconstitution by injecting human SCLC in the tail vein of severe combined immunodeficient (SCID) mice whereby the SCLC grows in the lung and other organs. Cisplatin (DDP) had significant antitumor effects on the SCLC growing orthotopically in the lung whereas mitomycin C (MMC) did not, thereby reflecting the clinical situation. However the opposite effects were found when the SCLC was growing subcutaneously, where the tumors responded to MMC and not to DDP. This suggests that the tumors growing orthotopically reflect the clinical effects of drugs on human SCLC more closely than the tumors growing subcutaneously. Therefore, this orthotopic reconstitution model of human SCLC in SCID mice is thought to be useful for studies on the treatment of human SCLC and emphasizes the need for orthotopic models for relevant cancer drug evaluation.

Original languageEnglish
Pages (from-to)627-630
Number of pages4
JournalAnticancer Research
Volume13
Issue number3
Publication statusPublished - 1993

Fingerprint

Drug Evaluation
SCID Mice
Small Cell Lung Carcinoma
Mitomycin
Neoplasms
Lung
Cisplatin
Tail
Veins

Keywords

  • Human small-cell lung carcinoma
  • Orthotopic transplant
  • SCID mouse
  • Site-specific cancer chemotherapy

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Site-specific chemosensitivity of human small-cell lung carcinoma growing orthotopically compared to subcutaneously in SCID mice : The importance of orthotopic models to obtain relevant drug evaluation data. / Kuo, T. H.; Kubota, T.; Watanabe, M.; Furukawa, Toshiharu; Kase, S.; Tanino, H.; Saikawa, Y.; Ishibiki, K.; Kitajima, M.; Hoffman, R. M.

In: Anticancer Research, Vol. 13, No. 3, 1993, p. 627-630.

Research output: Contribution to journalArticle

Kuo, T. H. ; Kubota, T. ; Watanabe, M. ; Furukawa, Toshiharu ; Kase, S. ; Tanino, H. ; Saikawa, Y. ; Ishibiki, K. ; Kitajima, M. ; Hoffman, R. M. / Site-specific chemosensitivity of human small-cell lung carcinoma growing orthotopically compared to subcutaneously in SCID mice : The importance of orthotopic models to obtain relevant drug evaluation data. In: Anticancer Research. 1993 ; Vol. 13, No. 3. pp. 627-630.
@article{72bf50f467194dc8861a72aa10e09833,
title = "Site-specific chemosensitivity of human small-cell lung carcinoma growing orthotopically compared to subcutaneously in SCID mice: The importance of orthotopic models to obtain relevant drug evaluation data",
abstract = "We have developed a novel in vivo model of human small-cell lung carcinoma (SCLC) using orthotopic reconstitution by injecting human SCLC in the tail vein of severe combined immunodeficient (SCID) mice whereby the SCLC grows in the lung and other organs. Cisplatin (DDP) had significant antitumor effects on the SCLC growing orthotopically in the lung whereas mitomycin C (MMC) did not, thereby reflecting the clinical situation. However the opposite effects were found when the SCLC was growing subcutaneously, where the tumors responded to MMC and not to DDP. This suggests that the tumors growing orthotopically reflect the clinical effects of drugs on human SCLC more closely than the tumors growing subcutaneously. Therefore, this orthotopic reconstitution model of human SCLC in SCID mice is thought to be useful for studies on the treatment of human SCLC and emphasizes the need for orthotopic models for relevant cancer drug evaluation.",
keywords = "Human small-cell lung carcinoma, Orthotopic transplant, SCID mouse, Site-specific cancer chemotherapy",
author = "Kuo, {T. H.} and T. Kubota and M. Watanabe and Toshiharu Furukawa and S. Kase and H. Tanino and Y. Saikawa and K. Ishibiki and M. Kitajima and Hoffman, {R. M.}",
year = "1993",
language = "English",
volume = "13",
pages = "627--630",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "3",

}

TY - JOUR

T1 - Site-specific chemosensitivity of human small-cell lung carcinoma growing orthotopically compared to subcutaneously in SCID mice

T2 - The importance of orthotopic models to obtain relevant drug evaluation data

AU - Kuo, T. H.

AU - Kubota, T.

AU - Watanabe, M.

AU - Furukawa, Toshiharu

AU - Kase, S.

AU - Tanino, H.

AU - Saikawa, Y.

AU - Ishibiki, K.

AU - Kitajima, M.

AU - Hoffman, R. M.

PY - 1993

Y1 - 1993

N2 - We have developed a novel in vivo model of human small-cell lung carcinoma (SCLC) using orthotopic reconstitution by injecting human SCLC in the tail vein of severe combined immunodeficient (SCID) mice whereby the SCLC grows in the lung and other organs. Cisplatin (DDP) had significant antitumor effects on the SCLC growing orthotopically in the lung whereas mitomycin C (MMC) did not, thereby reflecting the clinical situation. However the opposite effects were found when the SCLC was growing subcutaneously, where the tumors responded to MMC and not to DDP. This suggests that the tumors growing orthotopically reflect the clinical effects of drugs on human SCLC more closely than the tumors growing subcutaneously. Therefore, this orthotopic reconstitution model of human SCLC in SCID mice is thought to be useful for studies on the treatment of human SCLC and emphasizes the need for orthotopic models for relevant cancer drug evaluation.

AB - We have developed a novel in vivo model of human small-cell lung carcinoma (SCLC) using orthotopic reconstitution by injecting human SCLC in the tail vein of severe combined immunodeficient (SCID) mice whereby the SCLC grows in the lung and other organs. Cisplatin (DDP) had significant antitumor effects on the SCLC growing orthotopically in the lung whereas mitomycin C (MMC) did not, thereby reflecting the clinical situation. However the opposite effects were found when the SCLC was growing subcutaneously, where the tumors responded to MMC and not to DDP. This suggests that the tumors growing orthotopically reflect the clinical effects of drugs on human SCLC more closely than the tumors growing subcutaneously. Therefore, this orthotopic reconstitution model of human SCLC in SCID mice is thought to be useful for studies on the treatment of human SCLC and emphasizes the need for orthotopic models for relevant cancer drug evaluation.

KW - Human small-cell lung carcinoma

KW - Orthotopic transplant

KW - SCID mouse

KW - Site-specific cancer chemotherapy

UR - http://www.scopus.com/inward/record.url?scp=0027168844&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027168844&partnerID=8YFLogxK

M3 - Article

VL - 13

SP - 627

EP - 630

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 3

ER -