Sivelestat, a neutrophil elastase inhibitor, attenuates neutrophil priming after hepatoenteric ischemia in rabbits

Yoshifumi Kotake, Michiko Yamamoto, Midori Matsumoto, Hiroshi Morisaki, Junzo Takeda

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Neutrophils play an important role in ischemia-reperfusion injury. The neutrophil elastase not only causes tissue damage, but also mediates neutrophil priming. In the present study, we use a rabbit model of hepatoenteric ischemia-reperfusion to test the hypothesis that neutrophil elastase inhibition ameliorates an ischemia-reperfusion injury by attenuating neutrophil priming and suppressing enzymatic activity. Twenty-four Japanese white rabbits underwent 30 min of supraceliac aortic cross-clamping and 180 min of reperfusion under isoflurane anesthesia. The rabbits randomly received the neutrophil elastase inhibitor, sivelestat (n = 10), or saline (n = 14). Neutrophil priming was then assayed with luminol-dependent neutrophil chemiluminescence. Hepatic, intestinal, renal, and pulmonary damages were assessed with serum transaminase, lactate dehydrogenase concentrations, urinary N-acetyl glucosaminidase activity, and protein concentration in post mortem bronchoalveolar lavage fluid. We discovered that neutrophil elastase inhibition suppressed plasma neutrophil elastase, and that lipid peroxide concentrations increased after reperfusion. It improved ischemia-reperfusion injuries in the liver, intestine, kidney, and lung. Furthermore, inhibition of neutrophil elastase with sivelestat significantly attenuated post-reperfusion neutrophil priming. The results of this study demonstrate that neutrophil elastase inhibition could effectively attenuate an ischemia-reperfusion injury caused by supraceliac aortic cross-clamping, most likely from the attenuation of neutrophil priming.

Original languageEnglish
Pages (from-to)156-160
Number of pages5
JournalShock
Volume23
Issue number2
DOIs
Publication statusPublished - 2005 Feb

Fingerprint

Secretory Proteinase Inhibitory Proteins
Leukocyte Elastase
Neutrophils
Ischemia
Rabbits
Reperfusion Injury
Reperfusion
Constriction
Kidney
Hexosaminidases
Luminol
Lung
Lipid Peroxides
Liver
Isoflurane
Bronchoalveolar Lavage Fluid
Luminescence
Transaminases
sivelestat
L-Lactate Dehydrogenase

Keywords

  • Chemiluminescence
  • Kidney
  • Liver
  • Lung
  • Priming
  • Reperfusion

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Physiology

Cite this

Sivelestat, a neutrophil elastase inhibitor, attenuates neutrophil priming after hepatoenteric ischemia in rabbits. / Kotake, Yoshifumi; Yamamoto, Michiko; Matsumoto, Midori; Morisaki, Hiroshi; Takeda, Junzo.

In: Shock, Vol. 23, No. 2, 02.2005, p. 156-160.

Research output: Contribution to journalArticle

Kotake, Yoshifumi ; Yamamoto, Michiko ; Matsumoto, Midori ; Morisaki, Hiroshi ; Takeda, Junzo. / Sivelestat, a neutrophil elastase inhibitor, attenuates neutrophil priming after hepatoenteric ischemia in rabbits. In: Shock. 2005 ; Vol. 23, No. 2. pp. 156-160.
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