Size and Shape of Amyloid Fibrils Induced by Ganglioside Nanoclusters: Role of Sialyl Oligosaccharide in Fibril Formation

Teruhiko Matsubara, Masaya Nishihara, Hanaki Yasumori, Mako Nakai, Katsuhiko Yanagisawa, Toshinori Sato

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Ganglioside-enriched microdomains in the presynaptic neuronal membrane play a key role in the initiation of amyloid ß-protein (Aß) assembly related to Alzheimer's disease. We previously isolated lipids from a detergent-resistant membrane microdomain fraction of synaptosomes prepared from aged mouse brain and found that spherical Aß assemblies were formed on Aß-sensitive ganglioside nanoclusters (ASIGN) of reconstituted lipid bilayers in the synaptosomal fraction. In the present study, we investigated the role of oligosaccharides in Aß fibril formation induced by ganglioside-containing mixed lipid membranes that mimic the features of ASIGN. Ganglioside nanoclusters were constructed as ternary mixed lipid bilayers composed of ganglioside (GM1, GM2, GM3, GD1a, or GT1b), sphingomyelin, and cholesterol, and their surface topography was visualized by atomic force microscopy. Aß fibril formation on the nanocluster was strongly induced in the presence of 10 mol % ganglioside, and Aß-sensitive features were observed at cholesterol contents of 35-55 mol %. GM1-, GD1a-, and GT1b-containing membranes induced longer fibrils than those containing GD1b and GM2, indicating that the terminal galactose of GM1 along with N-acetylneuraminic acid accelerates protofibril elongation. These results demonstrate that Aß fibril formation is induced by ASIGN that are highly enriched ganglioside nanoclusters with a limited number of components and that the generation and elongation of Aß protofibrils are regulated by the oligosaccharide structure of gangliosides.

Original languageEnglish
Pages (from-to)13874-13881
Number of pages8
JournalLangmuir
Volume33
Issue number48
DOIs
Publication statusPublished - 2017 Dec 5

Fingerprint

Oligosaccharides
Gangliosides
Nanoclusters
nanoclusters
Amyloid
lipids
membranes
Lipid bilayers
Cholesterol
cholesterol
Membranes
elongation
Elongation
galactose
detergents
Detergents
Surface topography
Lipids
assemblies
brain

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Size and Shape of Amyloid Fibrils Induced by Ganglioside Nanoclusters : Role of Sialyl Oligosaccharide in Fibril Formation. / Matsubara, Teruhiko; Nishihara, Masaya; Yasumori, Hanaki; Nakai, Mako; Yanagisawa, Katsuhiko; Sato, Toshinori.

In: Langmuir, Vol. 33, No. 48, 05.12.2017, p. 13874-13881.

Research output: Contribution to journalArticle

Matsubara, Teruhiko ; Nishihara, Masaya ; Yasumori, Hanaki ; Nakai, Mako ; Yanagisawa, Katsuhiko ; Sato, Toshinori. / Size and Shape of Amyloid Fibrils Induced by Ganglioside Nanoclusters : Role of Sialyl Oligosaccharide in Fibril Formation. In: Langmuir. 2017 ; Vol. 33, No. 48. pp. 13874-13881.
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