Small-angle X-ray scattering study of adenosine triphosphatase from thermophilic bacterium PS3

Taiji Furuno, Akira Ikegami, Hiroshi Kihara, Masasuke Yoshida, Yasuo kagawa

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Adenosine triphosphatase from the thermophilic bacterium PS3(TF1) has been studied by solution X-ray scattering. A structural change in TF1 caused by the binding of ADP was observed by examining the difference between the radii of gyration of the unligated and ligated forms. The radius of gyration of the unligated TF1 was found to be 49·5±0·3 Å, and it decreased by approximately 3% after ligation with ADP. The positions and the amplitudes of a subsidiary maximum and a shoulder in the scattering profile showed subtle change on nucleotide binding. The lower limit of the maximum length of TF1 was determined to be 165 Å for the unligated form and 150 Å for the ligated form. The shape analysis of TF1 was performed by model calculations for simple triaxial bodies or their complexes. Among the various models tested, the one that gave the best fit with the experimental data consisted of seven ellipsoids of revolution; six identical ellipsoids with semi-axes: a = b = 18·5 Å and c = 74 Å, arranged hexagonally, and the other with a = b = 28 Å and c = 45 Å, located below the other six on the 6-fold axis. On the basis of this model it was suggested that there is a structural change on ligation with nucleotides, consisting of a shrinkage of the six long ellipsoids by 6% along their major axes.

Original languageEnglish
Pages (from-to)137-153
Number of pages17
JournalJournal of Molecular Biology
Volume170
Issue number1
DOIs
Publication statusPublished - 1983 Oct 15
Externally publishedYes

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Adenosine Diphosphate
Ligation
Adenosine Triphosphatases
Nucleotides
X-Rays
Bacteria

ASJC Scopus subject areas

  • Molecular Biology
  • Virology

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Small-angle X-ray scattering study of adenosine triphosphatase from thermophilic bacterium PS3. / Furuno, Taiji; Ikegami, Akira; Kihara, Hiroshi; Yoshida, Masasuke; kagawa, Yasuo.

In: Journal of Molecular Biology, Vol. 170, No. 1, 15.10.1983, p. 137-153.

Research output: Contribution to journalArticle

Furuno, Taiji ; Ikegami, Akira ; Kihara, Hiroshi ; Yoshida, Masasuke ; kagawa, Yasuo. / Small-angle X-ray scattering study of adenosine triphosphatase from thermophilic bacterium PS3. In: Journal of Molecular Biology. 1983 ; Vol. 170, No. 1. pp. 137-153.
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abstract = "Adenosine triphosphatase from the thermophilic bacterium PS3(TF1) has been studied by solution X-ray scattering. A structural change in TF1 caused by the binding of ADP was observed by examining the difference between the radii of gyration of the unligated and ligated forms. The radius of gyration of the unligated TF1 was found to be 49·5±0·3 {\AA}, and it decreased by approximately 3{\%} after ligation with ADP. The positions and the amplitudes of a subsidiary maximum and a shoulder in the scattering profile showed subtle change on nucleotide binding. The lower limit of the maximum length of TF1 was determined to be 165 {\AA} for the unligated form and 150 {\AA} for the ligated form. The shape analysis of TF1 was performed by model calculations for simple triaxial bodies or their complexes. Among the various models tested, the one that gave the best fit with the experimental data consisted of seven ellipsoids of revolution; six identical ellipsoids with semi-axes: a = b = 18·5 {\AA} and c = 74 {\AA}, arranged hexagonally, and the other with a = b = 28 {\AA} and c = 45 {\AA}, located below the other six on the 6-fold axis. On the basis of this model it was suggested that there is a structural change on ligation with nucleotides, consisting of a shrinkage of the six long ellipsoids by 6{\%} along their major axes.",
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