Small interfering RNA and gene silencing in transgenic mice and rats

Hidetoshi Hasuwa, Kazuhiro Kaseda, Thorbjorg Einarsdottir, Masaru Okabe

Research output: Contribution to journalArticle

217 Citations (Scopus)

Abstract

After short duplexes of synthetic 21-23 nt RNAs (siRNA) were reported to be effective in silencing specific genes, a vector-based approach for siRNAs was demonstrated in mammalian cultured cell lines. However, the effect of RNA interference (RNAi) on various differentiated cells in live animals remains unknown. In this report, we demonstrate that transgenically supplied siRNA can silence ubiquitously expressed enhanced green fluorescent protein in every part of the mouse and rat body. These results suggest that transgenic RNAi could function as an alternative method of gene silencing by applying homologous recombination to embryonic stem (ES) cells, and should be successful even in species where ES cell lines remain unestablished.

Original languageEnglish
Pages (from-to)227-230
Number of pages4
JournalFEBS Letters
Volume532
Issue number1-2
DOIs
Publication statusPublished - 2002 Dec 4
Externally publishedYes

Fingerprint

Transgenic Rats
Gene Silencing
Embryonic Stem Cells
RNA Interference
Small Interfering RNA
Transgenic Mice
Rats
Genes
RNA
Cell Line
Homologous Recombination
Stem cells
Cultured Cells
Animals
Cells
enhanced green fluorescent protein

Keywords

  • Gene silencing
  • RNA interference
  • RNA polymerase III promoter
  • Small interfering RNA
  • Transgenic animal

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Small interfering RNA and gene silencing in transgenic mice and rats. / Hasuwa, Hidetoshi; Kaseda, Kazuhiro; Einarsdottir, Thorbjorg; Okabe, Masaru.

In: FEBS Letters, Vol. 532, No. 1-2, 04.12.2002, p. 227-230.

Research output: Contribution to journalArticle

Hasuwa, Hidetoshi ; Kaseda, Kazuhiro ; Einarsdottir, Thorbjorg ; Okabe, Masaru. / Small interfering RNA and gene silencing in transgenic mice and rats. In: FEBS Letters. 2002 ; Vol. 532, No. 1-2. pp. 227-230.
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