TY - JOUR
T1 - Small interfering RNA and gene silencing in transgenic mice and rats
AU - Hasuwa, Hidetoshi
AU - Kaseda, Kazuhiro
AU - Einarsdottir, Thorbjorg
AU - Okabe, Masaru
N1 - Funding Information:
We thank Stuart B. Moss for critically reading the manuscript. This work was supported by grants from the Ministry of Education, Science, Sports, and Culture (14780625, 11234203 and 14380383).
PY - 2002/12/4
Y1 - 2002/12/4
N2 - After short duplexes of synthetic 21-23 nt RNAs (siRNA) were reported to be effective in silencing specific genes, a vector-based approach for siRNAs was demonstrated in mammalian cultured cell lines. However, the effect of RNA interference (RNAi) on various differentiated cells in live animals remains unknown. In this report, we demonstrate that transgenically supplied siRNA can silence ubiquitously expressed enhanced green fluorescent protein in every part of the mouse and rat body. These results suggest that transgenic RNAi could function as an alternative method of gene silencing by applying homologous recombination to embryonic stem (ES) cells, and should be successful even in species where ES cell lines remain unestablished.
AB - After short duplexes of synthetic 21-23 nt RNAs (siRNA) were reported to be effective in silencing specific genes, a vector-based approach for siRNAs was demonstrated in mammalian cultured cell lines. However, the effect of RNA interference (RNAi) on various differentiated cells in live animals remains unknown. In this report, we demonstrate that transgenically supplied siRNA can silence ubiquitously expressed enhanced green fluorescent protein in every part of the mouse and rat body. These results suggest that transgenic RNAi could function as an alternative method of gene silencing by applying homologous recombination to embryonic stem (ES) cells, and should be successful even in species where ES cell lines remain unestablished.
KW - Gene silencing
KW - RNA interference
KW - RNA polymerase III promoter
KW - Small interfering RNA
KW - Transgenic animal
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U2 - 10.1016/S0014-5793(02)03680-3
DO - 10.1016/S0014-5793(02)03680-3
M3 - Article
C2 - 12459495
AN - SCOPUS:0037021437
SN - 0014-5793
VL - 532
SP - 227
EP - 230
JO - FEBS Letters
JF - FEBS Letters
IS - 1-2
ER -