Small molecules assisting eggshell calcium dissolution for embryonic bone formation

Taku Ito, Suguru Kato, Akiko Kubo, Makoto Suematsu, Masaya Nakata, Yoko Saikawa

Research output: Contribution to journalArticle

Abstract

Calcium carbonate in avian eggshell is used as a calcium source for bone formation during embryonic development. We studied ostrich eggshells as a representative avian egg and found that the calcium reserve assembly (CRA) region, eventually dissolving for bone formation, consists of nano-granules comprising amorphous calcium carbonate (ACC) by careful microscopic observation. ACC is unstable phase of calcium carbonate with high solubility and is stabilized by additives. As such a stabilizer, we found small molecules from eggshell internal region. Particularly, the ACC was recreated by the formation of calcium carbonate in the presence of D-myo-inositol 4,5-bisphosphate (Ins(4,5)P2), isolated from ostrich eggshell for the first time in this study. Further mass spectrometric imaging technique revealed that Ins(4,5)P2 is localized at the CRA region, suggesting that this compound could play a major role that makes it easier to dissolve and internalize eggshell calcium during embryogenesis.

Original languageEnglish
Article number130854
JournalTetrahedron
DOIs
Publication statusAccepted/In press - 2019 Jan 1

Fingerprint

Egg Shell
Calcium Carbonate
Osteogenesis
Bone
Dissolution
Calcium
Molecules
Struthioniformes
Embryonic Development
Solubility
Ovum
Imaging techniques
inositol 4,5-bisphosphate

Keywords

  • Amorphous calcium carbonate
  • Eggshell
  • Embryogenesis
  • Inositol phosphate

ASJC Scopus subject areas

  • Biochemistry
  • Drug Discovery
  • Organic Chemistry

Cite this

Small molecules assisting eggshell calcium dissolution for embryonic bone formation. / Ito, Taku; Kato, Suguru; Kubo, Akiko; Suematsu, Makoto; Nakata, Masaya; Saikawa, Yoko.

In: Tetrahedron, 01.01.2019.

Research output: Contribution to journalArticle

@article{6de277f03a5b4805acb4fde756dda623,
title = "Small molecules assisting eggshell calcium dissolution for embryonic bone formation",
abstract = "Calcium carbonate in avian eggshell is used as a calcium source for bone formation during embryonic development. We studied ostrich eggshells as a representative avian egg and found that the calcium reserve assembly (CRA) region, eventually dissolving for bone formation, consists of nano-granules comprising amorphous calcium carbonate (ACC) by careful microscopic observation. ACC is unstable phase of calcium carbonate with high solubility and is stabilized by additives. As such a stabilizer, we found small molecules from eggshell internal region. Particularly, the ACC was recreated by the formation of calcium carbonate in the presence of D-myo-inositol 4,5-bisphosphate (Ins(4,5)P2), isolated from ostrich eggshell for the first time in this study. Further mass spectrometric imaging technique revealed that Ins(4,5)P2 is localized at the CRA region, suggesting that this compound could play a major role that makes it easier to dissolve and internalize eggshell calcium during embryogenesis.",
keywords = "Amorphous calcium carbonate, Eggshell, Embryogenesis, Inositol phosphate",
author = "Taku Ito and Suguru Kato and Akiko Kubo and Makoto Suematsu and Masaya Nakata and Yoko Saikawa",
year = "2019",
month = "1",
day = "1",
doi = "10.1016/j.tet.2019.130854",
language = "English",
journal = "Tetrahedron",
issn = "0040-4020",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Small molecules assisting eggshell calcium dissolution for embryonic bone formation

AU - Ito, Taku

AU - Kato, Suguru

AU - Kubo, Akiko

AU - Suematsu, Makoto

AU - Nakata, Masaya

AU - Saikawa, Yoko

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Calcium carbonate in avian eggshell is used as a calcium source for bone formation during embryonic development. We studied ostrich eggshells as a representative avian egg and found that the calcium reserve assembly (CRA) region, eventually dissolving for bone formation, consists of nano-granules comprising amorphous calcium carbonate (ACC) by careful microscopic observation. ACC is unstable phase of calcium carbonate with high solubility and is stabilized by additives. As such a stabilizer, we found small molecules from eggshell internal region. Particularly, the ACC was recreated by the formation of calcium carbonate in the presence of D-myo-inositol 4,5-bisphosphate (Ins(4,5)P2), isolated from ostrich eggshell for the first time in this study. Further mass spectrometric imaging technique revealed that Ins(4,5)P2 is localized at the CRA region, suggesting that this compound could play a major role that makes it easier to dissolve and internalize eggshell calcium during embryogenesis.

AB - Calcium carbonate in avian eggshell is used as a calcium source for bone formation during embryonic development. We studied ostrich eggshells as a representative avian egg and found that the calcium reserve assembly (CRA) region, eventually dissolving for bone formation, consists of nano-granules comprising amorphous calcium carbonate (ACC) by careful microscopic observation. ACC is unstable phase of calcium carbonate with high solubility and is stabilized by additives. As such a stabilizer, we found small molecules from eggshell internal region. Particularly, the ACC was recreated by the formation of calcium carbonate in the presence of D-myo-inositol 4,5-bisphosphate (Ins(4,5)P2), isolated from ostrich eggshell for the first time in this study. Further mass spectrometric imaging technique revealed that Ins(4,5)P2 is localized at the CRA region, suggesting that this compound could play a major role that makes it easier to dissolve and internalize eggshell calcium during embryogenesis.

KW - Amorphous calcium carbonate

KW - Eggshell

KW - Embryogenesis

KW - Inositol phosphate

UR - http://www.scopus.com/inward/record.url?scp=85076860057&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85076860057&partnerID=8YFLogxK

U2 - 10.1016/j.tet.2019.130854

DO - 10.1016/j.tet.2019.130854

M3 - Article

AN - SCOPUS:85076860057

JO - Tetrahedron

JF - Tetrahedron

SN - 0040-4020

M1 - 130854

ER -