Small RNA profiling and characterization of piRNA clusters in the adult testes of the common marmoset, a model primate

Takamasa Hirano, Yuka Iwasaki, Zachary Yu Ching Lin, Masanori Imamura, Naomi M. Seki, Erika Sasaki, Kuniaki Saito, Hideyuki Okano, Mikiko C. Siomi, Haruhiko Siomi

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Small RNAs mediate gene silencing by binding Argonaute/Piwi proteins to regulate target RNAs. Here, we describe small RNA profiling of the adult testes of Callithrix jacchus, the common marmoset. The most abundant class of small RNAs in the adult testis was piRNAs, although 353 novel miRNAs but few endo-siRNAs were also identified. MARWI, a marmoset homolog of mouse MIWI and a very abundant PIWI in adult testes, associates with piRNAs that show characteristics of mouse pachytene piRNAs. As in other mammals, most marmoset piRNAs are derived from conserved clustered regions in the genome, which are annotated as intergenic regions. However, unlike in mice, marmoset piRNA clusters are also found on the X chromosome, suggesting escape from meiotic sex chromosome inactivation by the X-linked clusters. Some of the piRNA clusters identified contain antisense-orientated pseudogenes, suggesting the possibility that pseudogene-derived piRNAs may regulate parental functional protein-coding genes. More piRNAs map to transposable element (TE) subfamilies when they have copies in piRNA clusters. In addition, the strand bias observed for piRNAs mapped to each TE subfamily correlates with the polarity of copies inserted in clusters. These findings suggest that pachytene piRNA clusters determine the abundance and strand-bias of TE-derived piRNAs, may regulate protein-coding genes via pseudogene-derived piRNAs, and may even play roles in meiosis in the adult marmoset testis.

Original languageEnglish
Pages (from-to)1223-1237
Number of pages15
JournalRNA
Volume20
Issue number8
DOIs
Publication statusPublished - 2014

Fingerprint

Callithrix
Primates
Small Interfering RNA
Testis
RNA
Pseudogenes
DNA Transposable Elements
Argonaute Proteins
X Chromosome Inactivation
Sex Chromosomes
Intergenic DNA
Meiosis
X Chromosome
Gene Silencing
MicroRNAs
Mammals
Carrier Proteins
Proteins
Genome

Keywords

  • miRNAs
  • piRNAs
  • PIWI
  • Pseudogenes
  • Transposable elements

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Small RNA profiling and characterization of piRNA clusters in the adult testes of the common marmoset, a model primate. / Hirano, Takamasa; Iwasaki, Yuka; Lin, Zachary Yu Ching; Imamura, Masanori; Seki, Naomi M.; Sasaki, Erika; Saito, Kuniaki; Okano, Hideyuki; Siomi, Mikiko C.; Siomi, Haruhiko.

In: RNA, Vol. 20, No. 8, 2014, p. 1223-1237.

Research output: Contribution to journalArticle

Hirano, Takamasa ; Iwasaki, Yuka ; Lin, Zachary Yu Ching ; Imamura, Masanori ; Seki, Naomi M. ; Sasaki, Erika ; Saito, Kuniaki ; Okano, Hideyuki ; Siomi, Mikiko C. ; Siomi, Haruhiko. / Small RNA profiling and characterization of piRNA clusters in the adult testes of the common marmoset, a model primate. In: RNA. 2014 ; Vol. 20, No. 8. pp. 1223-1237.
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