"Smart Eye Camera"

An innovative technique to evaluate tear film breakup time in a murine dry eye disease model

Eisuke Shimizu, Yoko Ogawa, Hiroyuki Yazu, Naohiko Aketa, Fan Yang, Mio Yamane, Yasunori Sato, Yutaka Kawakami, Kazuo Tsubota

Research output: Contribution to journalArticle

Abstract

Tear film breakup time (TFBUT) is an essential parameter used to diagnose dry eye disease (DED). However, a robust method for examining TFBUT in murine models has yet to be established. We invented an innovative device, namely, the "Smart Eye Camera", which addresses several problems associated with existing methods and is capable of evaluating TFBUT in a murine DED model. We compared images taken by existing devices and the Smart Eye Camera in a graft-versus-host disease-related DED murine model. We observed that the quality of the images obtained by the Smart Eye Camera were sufficient for practical use. Moreover, this new technique could be used to obtain measurements for several consecutive ocular phenotypes in a variety of environments. Here, we demonstrate the effectiveness of our new invention in the examination of ocular phenotypes, including TFBUT in a murine model. We highlight the potential for future translational studies adopting the Smart Eye Camera in clinical settings.

Original languageEnglish
Article numbere0215130
JournalPloS one
Volume14
Issue number5
DOIs
Publication statusPublished - 2019 May 1

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eye diseases
Eye Diseases
disease models
Tears
cameras
eyes
Cameras
mice
animal models
Patents and inventions
methodology
Grafts
Phenotype
phenotype
Equipment and Supplies
Graft vs Host Disease

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

"Smart Eye Camera" : An innovative technique to evaluate tear film breakup time in a murine dry eye disease model. / Shimizu, Eisuke; Ogawa, Yoko; Yazu, Hiroyuki; Aketa, Naohiko; Yang, Fan; Yamane, Mio; Sato, Yasunori; Kawakami, Yutaka; Tsubota, Kazuo.

In: PloS one, Vol. 14, No. 5, e0215130, 01.05.2019.

Research output: Contribution to journalArticle

Shimizu, E, Ogawa, Y, Yazu, H, Aketa, N, Yang, F, Yamane, M, Sato, Y, Kawakami, Y & Tsubota, K 2019, '"Smart Eye Camera": An innovative technique to evaluate tear film breakup time in a murine dry eye disease model', PloS one, vol. 14, no. 5, e0215130. https://doi.org/10.1371/journal.pone.021513
Shimizu, Eisuke ; Ogawa, Yoko ; Yazu, Hiroyuki ; Aketa, Naohiko ; Yang, Fan ; Yamane, Mio ; Sato, Yasunori ; Kawakami, Yutaka ; Tsubota, Kazuo. / "Smart Eye Camera" : An innovative technique to evaluate tear film breakup time in a murine dry eye disease model. In: PloS one. 2019 ; Vol. 14, No. 5.
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