Abstract
We here report a new culturing method to produce micro vascular-mimetic tissue consisting of smooth muscle cells (SMCs) and endothelial cells (ECs) toward the practical use of tissue engineering. To achieve the stable coculture, the requirements for SMC culture in microchannels are clarified by basic experiments. Then, a new microchip that satisfies those requirements was developed by bonding glass substrate and porous polycarbonate membranes. Finally, the stable coculture of SMC and ECs in confined micro-space was demonstrated for the first time. Those knowledge and new system would contribute not only to the vascular tissue engineering but also to the cellular engineering in micro-space.
| Original language | English |
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| Title of host publication | Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1708-1710 |
| Number of pages | 3 |
| ISBN (Print) | 9780979806452 |
| Publication status | Published - 2012 Jan 1 |
| Externally published | Yes |
| Event | 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan Duration: 2012 Oct 28 → 2012 Nov 1 |
Publication series
| Name | Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
|---|
Other
| Other | 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
|---|---|
| Country | Japan |
| City | Okinawa |
| Period | 12/10/28 → 12/11/1 |
Keywords
- Curvature
- Smooth muscle cell
- Vascular tissue
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering
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Cite this
Yamashita, T., Mawatari, K., Tanaka, Y., & Kitamori, T. (2012). Smooth muscle cell culture in microchannel toward construction of multilayered vascular tissue in micro-scale. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1708-1710). (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012). Chemical and Biological Microsystems Society.