Abstract
We here report a new culturing method to produce micro vascular-mimetic tissue consisting of smooth muscle cells (SMCs) and endothelial cells (ECs) toward the practical use of tissue engineering. To achieve the stable coculture, the requirements for SMC culture in microchannels are clarified by basic experiments. Then, a new microchip that satisfies those requirements was developed by bonding glass substrate and porous polycarbonate membranes. Finally, the stable coculture of SMC and ECs in confined micro-space was demonstrated for the first time. Those knowledge and new system would contribute not only to the vascular tissue engineering but also to the cellular engineering in micro-space.
| Original language | English |
|---|---|
| Title of host publication | Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1708-1710 |
| Number of pages | 3 |
| ISBN (Print) | 9780979806452 |
| Publication status | Published - 2012 Jan 1 |
| Externally published | Yes |
| Event | 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan Duration: 2012 Oct 28 → 2012 Nov 1 |
Other
| Other | 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
|---|---|
| Country | Japan |
| City | Okinawa |
| Period | 12/10/28 → 12/11/1 |
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Keywords
- Curvature
- Smooth muscle cell
- Vascular tissue
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering
Cite this
Smooth muscle cell culture in microchannel toward construction of multilayered vascular tissue in micro-scale. / Yamashita, Tadahiro; Mawatari, Kazuma; Tanaka, Yo; Kitamori, Takehiko.
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 1708-1710.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Smooth muscle cell culture in microchannel toward construction of multilayered vascular tissue in micro-scale
AU - Yamashita, Tadahiro
AU - Mawatari, Kazuma
AU - Tanaka, Yo
AU - Kitamori, Takehiko
PY - 2012/1/1
Y1 - 2012/1/1
N2 - We here report a new culturing method to produce micro vascular-mimetic tissue consisting of smooth muscle cells (SMCs) and endothelial cells (ECs) toward the practical use of tissue engineering. To achieve the stable coculture, the requirements for SMC culture in microchannels are clarified by basic experiments. Then, a new microchip that satisfies those requirements was developed by bonding glass substrate and porous polycarbonate membranes. Finally, the stable coculture of SMC and ECs in confined micro-space was demonstrated for the first time. Those knowledge and new system would contribute not only to the vascular tissue engineering but also to the cellular engineering in micro-space.
AB - We here report a new culturing method to produce micro vascular-mimetic tissue consisting of smooth muscle cells (SMCs) and endothelial cells (ECs) toward the practical use of tissue engineering. To achieve the stable coculture, the requirements for SMC culture in microchannels are clarified by basic experiments. Then, a new microchip that satisfies those requirements was developed by bonding glass substrate and porous polycarbonate membranes. Finally, the stable coculture of SMC and ECs in confined micro-space was demonstrated for the first time. Those knowledge and new system would contribute not only to the vascular tissue engineering but also to the cellular engineering in micro-space.
KW - Curvature
KW - Smooth muscle cell
KW - Vascular tissue
UR - http://www.scopus.com/inward/record.url?scp=84901777054&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901777054&partnerID=8YFLogxK
M3 - Conference contribution
SN - 9780979806452
SP - 1708
EP - 1710
BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PB - Chemical and Biological Microsystems Society
ER -