TY - GEN
T1 - Smooth muscle cell culture in microchannel toward construction of multilayered vascular tissue in micro-scale
AU - Yamashita, Tadahiro
AU - Mawatari, Kazuma
AU - Tanaka, Yo
AU - Kitamori, Takehiko
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2012
Y1 - 2012
N2 - We here report a new culturing method to produce micro vascular-mimetic tissue consisting of smooth muscle cells (SMCs) and endothelial cells (ECs) toward the practical use of tissue engineering. To achieve the stable coculture, the requirements for SMC culture in microchannels are clarified by basic experiments. Then, a new microchip that satisfies those requirements was developed by bonding glass substrate and porous polycarbonate membranes. Finally, the stable coculture of SMC and ECs in confined micro-space was demonstrated for the first time. Those knowledge and new system would contribute not only to the vascular tissue engineering but also to the cellular engineering in micro-space.
AB - We here report a new culturing method to produce micro vascular-mimetic tissue consisting of smooth muscle cells (SMCs) and endothelial cells (ECs) toward the practical use of tissue engineering. To achieve the stable coculture, the requirements for SMC culture in microchannels are clarified by basic experiments. Then, a new microchip that satisfies those requirements was developed by bonding glass substrate and porous polycarbonate membranes. Finally, the stable coculture of SMC and ECs in confined micro-space was demonstrated for the first time. Those knowledge and new system would contribute not only to the vascular tissue engineering but also to the cellular engineering in micro-space.
KW - Curvature
KW - Smooth muscle cell
KW - Vascular tissue
UR - http://www.scopus.com/inward/record.url?scp=84901777054&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901777054&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901777054
SN - 9780979806452
T3 - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
SP - 1708
EP - 1710
BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PB - Chemical and Biological Microsystems Society
T2 - 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Y2 - 28 October 2012 through 1 November 2012
ER -