Smooth muscle cell culture in microchannel toward construction of multilayered vascular tissue in micro-scale

Tadahiro Yamashita, Kazuma Mawatari, Yo Tanaka, Takehiko Kitamori

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We here report a new culturing method to produce micro vascular-mimetic tissue consisting of smooth muscle cells (SMCs) and endothelial cells (ECs) toward the practical use of tissue engineering. To achieve the stable coculture, the requirements for SMC culture in microchannels are clarified by basic experiments. Then, a new microchip that satisfies those requirements was developed by bonding glass substrate and porous polycarbonate membranes. Finally, the stable coculture of SMC and ECs in confined micro-space was demonstrated for the first time. Those knowledge and new system would contribute not only to the vascular tissue engineering but also to the cellular engineering in micro-space.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1708-1710
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012 Jan 1
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 2012 Oct 282012 Nov 1

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12/10/2812/11/1

Fingerprint

Microchannels
Cell culture
Muscle
polycarbonate
Endothelial cells
Tissue
Tissue engineering
Glass bonding
Polycarbonates
Cells
Membranes
Substrates
Experiments

Keywords

  • Curvature
  • Smooth muscle cell
  • Vascular tissue

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Yamashita, T., Mawatari, K., Tanaka, Y., & Kitamori, T. (2012). Smooth muscle cell culture in microchannel toward construction of multilayered vascular tissue in micro-scale. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1708-1710). Chemical and Biological Microsystems Society.

Smooth muscle cell culture in microchannel toward construction of multilayered vascular tissue in micro-scale. / Yamashita, Tadahiro; Mawatari, Kazuma; Tanaka, Yo; Kitamori, Takehiko.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 1708-1710.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yamashita, T, Mawatari, K, Tanaka, Y & Kitamori, T 2012, Smooth muscle cell culture in microchannel toward construction of multilayered vascular tissue in micro-scale. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, pp. 1708-1710, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 12/10/28.
Yamashita T, Mawatari K, Tanaka Y, Kitamori T. Smooth muscle cell culture in microchannel toward construction of multilayered vascular tissue in micro-scale. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 1708-1710
Yamashita, Tadahiro ; Mawatari, Kazuma ; Tanaka, Yo ; Kitamori, Takehiko. / Smooth muscle cell culture in microchannel toward construction of multilayered vascular tissue in micro-scale. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 1708-1710
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