SOCS3 in T and NKT cells negatively regulates cytokine production and ameliorates ConA-induced hepatitis

Mako Nakaya, Masayuki Hashimoto, Ryusuke Nakagawa, Yu Wakabayashi, Takuma Ishizaki, Ichiro Takada, Kyoko Komai, Hiroki Yoshida, Akihiko Yoshimura

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Abstract

Suppressor of cytokine signaling 3 (SOCS3), a negative-feedback molecule for cytokine signaling, has been implicated in protection against liver injury. Previous studies have shown that overexpression of SOCS3 in the liver by adenovirus or membrane permeable recombinant protein protected the liver from various injuries. However it remained uncertain in which type of cells SOCS3 suppresses liver injury. In this study, we demonstrated that forced expression of SOCS3 in T and NKT cells suppressed ConA-induced hepatitis using T and NKT cell-specific SOCS3 transgenic (Lck-SOCS3 Tg) mice. IFN-γ and IL-4 production was reduced in Lck-SOCS3 Tg mice as well as splenocytes treated with ConA. IFN-γ and IL-4 levels were also reduced in Lck-SOCS3 Tg mice administrated with α-galactosylceramide, suggesting that SOCS3 in NKT cells has suppressive function. Sustained expression of SOCS3 in an NKT cell line also resulted in reduced expression of various cytokines and transcription factors. In contrast, T and NKT cell-specific SOCS3 conditional knockout (Lck-SOCS3 cKO) mice were hypersensitive to ConA-mediated hepatitis. Isolated SOCS3-deficient NKT cells produced higher levels of IFN-γ and IL-4. These data indicate that SOCS3 plays a negative regulatory role in NKT cell activation and that forced expression of SOCS3 in NKT cells is effective in preventing hepatitis.

Original languageEnglish
Pages (from-to)7047-7053
Number of pages7
JournalJournal of Immunology
Volume183
Issue number11
DOIs
Publication statusPublished - 2009 Dec 1

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Natural Killer T-Cells
Hepatitis
Cytokines
T-Lymphocytes
Interleukin-4
Liver
Wounds and Injuries
Galactosylceramides

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

SOCS3 in T and NKT cells negatively regulates cytokine production and ameliorates ConA-induced hepatitis. / Nakaya, Mako; Hashimoto, Masayuki; Nakagawa, Ryusuke; Wakabayashi, Yu; Ishizaki, Takuma; Takada, Ichiro; Komai, Kyoko; Yoshida, Hiroki; Yoshimura, Akihiko.

In: Journal of Immunology, Vol. 183, No. 11, 01.12.2009, p. 7047-7053.

Research output: Contribution to journalArticle

Nakaya, M, Hashimoto, M, Nakagawa, R, Wakabayashi, Y, Ishizaki, T, Takada, I, Komai, K, Yoshida, H & Yoshimura, A 2009, 'SOCS3 in T and NKT cells negatively regulates cytokine production and ameliorates ConA-induced hepatitis', Journal of Immunology, vol. 183, no. 11, pp. 7047-7053. https://doi.org/10.4049/jimmunol.0900547
Nakaya M, Hashimoto M, Nakagawa R, Wakabayashi Y, Ishizaki T, Takada I et al. SOCS3 in T and NKT cells negatively regulates cytokine production and ameliorates ConA-induced hepatitis. Journal of Immunology. 2009 Dec 1;183(11):7047-7053. https://doi.org/10.4049/jimmunol.0900547
Nakaya, Mako ; Hashimoto, Masayuki ; Nakagawa, Ryusuke ; Wakabayashi, Yu ; Ishizaki, Takuma ; Takada, Ichiro ; Komai, Kyoko ; Yoshida, Hiroki ; Yoshimura, Akihiko. / SOCS3 in T and NKT cells negatively regulates cytokine production and ameliorates ConA-induced hepatitis. In: Journal of Immunology. 2009 ; Vol. 183, No. 11. pp. 7047-7053.
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