SOCS3 regulates p21 expression and cell cycle arrest in response to DNA damage

John C. Sitko, Brian Yeh, Moonhong Kim, Hong Zhou, Giichi Takaesu, Akihiko Yoshimura, William H. McBride, Anahid Jewett, Christina A M Jamieson, Nicholas A. Cacalano

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Genotoxic agents such as ionizing radiation trigger cell cycle arrest at the G1/S and G2/M checkpoints, allowing cells to repair damaged DNA before entry into mitosis. DNA damage-induced G1 arrest involves p53-dependent expression of p21 (Cip1/Waf-1), which inhibits cyclin-dependent kinases and blocks S phase entry. While much of the core DNA damage response has been well-studied, other signaling proteins that intersect with and modulate this response remain uncharacterized. In this study, we identify Suppressor of Cytokine Signaling (SOCS)-3 as an important regulator of radiation-induced G1 arrest. SOCS3-deficient fibroblasts fail to undergo G1 arrest and accumulate in the G2/M phase of the cell cycle. SOCS3 knockout cells phosphorylate p53 and H2AX normally in response to radiation, but fail to upregulate p21 expression. In addition, STAT3 phosphorylation is elevated in SOCS3-deficient cells compared to WT cells. Normal G1 arrest can be restored in SOCS3 KO cells by retroviral transduction of WT SOCS3 or a dominant-negative mutant of STAT3. Our results suggest a novel function for SOCS3 in the control of genome stability by negatively regulating STAT3-dependent radioresistant DNA synthesis, and promoting p53-dependent p21 expression.

Original languageEnglish
Pages (from-to)2221-2230
Number of pages10
JournalCellular Signalling
Volume20
Issue number12
DOIs
Publication statusPublished - 2008 Dec
Externally publishedYes

Fingerprint

Cell Cycle Checkpoints
DNA Damage
Radiation
G1 Phase Cell Cycle Checkpoints
Cyclin-Dependent Kinases
Genomic Instability
G2 Phase
Ionizing Radiation
S Phase
Mitosis
DNA Repair
Cell Division
Cell Cycle
Up-Regulation
Fibroblasts
Phosphorylation
Cytokines
DNA
Proteins

Keywords

  • Cell cycle
  • DNA damage
  • p21
  • Signaling
  • SOCS3
  • STAT3

ASJC Scopus subject areas

  • Cell Biology

Cite this

Sitko, J. C., Yeh, B., Kim, M., Zhou, H., Takaesu, G., Yoshimura, A., ... Cacalano, N. A. (2008). SOCS3 regulates p21 expression and cell cycle arrest in response to DNA damage. Cellular Signalling, 20(12), 2221-2230. https://doi.org/10.1016/j.cellsig.2008.08.011

SOCS3 regulates p21 expression and cell cycle arrest in response to DNA damage. / Sitko, John C.; Yeh, Brian; Kim, Moonhong; Zhou, Hong; Takaesu, Giichi; Yoshimura, Akihiko; McBride, William H.; Jewett, Anahid; Jamieson, Christina A M; Cacalano, Nicholas A.

In: Cellular Signalling, Vol. 20, No. 12, 12.2008, p. 2221-2230.

Research output: Contribution to journalArticle

Sitko, JC, Yeh, B, Kim, M, Zhou, H, Takaesu, G, Yoshimura, A, McBride, WH, Jewett, A, Jamieson, CAM & Cacalano, NA 2008, 'SOCS3 regulates p21 expression and cell cycle arrest in response to DNA damage', Cellular Signalling, vol. 20, no. 12, pp. 2221-2230. https://doi.org/10.1016/j.cellsig.2008.08.011
Sitko, John C. ; Yeh, Brian ; Kim, Moonhong ; Zhou, Hong ; Takaesu, Giichi ; Yoshimura, Akihiko ; McBride, William H. ; Jewett, Anahid ; Jamieson, Christina A M ; Cacalano, Nicholas A. / SOCS3 regulates p21 expression and cell cycle arrest in response to DNA damage. In: Cellular Signalling. 2008 ; Vol. 20, No. 12. pp. 2221-2230.
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