Soft-hydrothermal processing of red cedar bedding reduces its induction of cytochrome P450 in mouse liver

Z. Li, S. Okano, K. Yoshinari, T. Miyamoto, Y. Yamazoe, K. Shinya, Koji Ioku, N. Kasai

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Red cedar-derived bedding materials cause changes in cytochrome P450-dependent microsomal enzyme systems in laboratory animals. We examined the effect of essential oil of red cedar (EORC), as well as the effect of bedding from which it had been removed, on the hepatic expression cytochrome P450s in mice. EORC was obtained from liquid extracts of red cedar bedding by a soft-hydrothermal process and was administered orally to mice. Between days 1 and 2 after administration, hepatic P450s were significantly induced as follows: CYP3As, 7.1 ×; CYP1As, 1.6×; CYP2E1, 1.5×; CYP2Cs, 1.6×. A housing study of mice indicated that red cedar bedding increased the levels of these P450s in mouse liver, whereas mice housed in cedar bedding from which EORC had been removed (ST-cedar bedding) showed significantly lower levels of P450s, especially CYP3As, CYP1As and CYP2E1. Soft-hydrothermal processing partially removed many components of EORC. In particular, several volatile sesquiterpenes, naphthalene-derived aromatics and 4,4-dimethyl- 13α-androst-5-ene were decreased in the ST-cedar bedding, suggesting that these may be responsible for P450 induction. This study demonstrated that the removal of these volatile compounds by soft-hydrothermal processing can decrease the hepatic P450-inducing effect of red cedar bedding.

Original languageEnglish
Pages (from-to)205-211
Number of pages7
JournalLaboratory Animals
Volume43
Issue number2
DOIs
Publication statusPublished - 2009 Apr
Externally publishedYes

Fingerprint

cytochrome P-450
Cytochrome P-450 Enzyme System
Volatile Oils
essential oils
liver
Liver
mice
Cytochrome P-450 CYP3A
Cytochrome P-450 CYP2E1
naphthalene
Sesquiterpenes
Laboratory Animals
Cytochromes
cytochromes
sesquiterpenoids
volatile compounds
laboratory animals
aromatic compounds
liquids
extracts

Keywords

  • Cytochrome P450 induction
  • Essential oil
  • Mouse liver
  • Red cedar bedding
  • Soft-hydrothermal processing

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Li, Z., Okano, S., Yoshinari, K., Miyamoto, T., Yamazoe, Y., Shinya, K., ... Kasai, N. (2009). Soft-hydrothermal processing of red cedar bedding reduces its induction of cytochrome P450 in mouse liver. Laboratory Animals, 43(2), 205-211. https://doi.org/10.1258/la.2008.007146

Soft-hydrothermal processing of red cedar bedding reduces its induction of cytochrome P450 in mouse liver. / Li, Z.; Okano, S.; Yoshinari, K.; Miyamoto, T.; Yamazoe, Y.; Shinya, K.; Ioku, Koji; Kasai, N.

In: Laboratory Animals, Vol. 43, No. 2, 04.2009, p. 205-211.

Research output: Contribution to journalArticle

Li, Z, Okano, S, Yoshinari, K, Miyamoto, T, Yamazoe, Y, Shinya, K, Ioku, K & Kasai, N 2009, 'Soft-hydrothermal processing of red cedar bedding reduces its induction of cytochrome P450 in mouse liver', Laboratory Animals, vol. 43, no. 2, pp. 205-211. https://doi.org/10.1258/la.2008.007146
Li, Z. ; Okano, S. ; Yoshinari, K. ; Miyamoto, T. ; Yamazoe, Y. ; Shinya, K. ; Ioku, Koji ; Kasai, N. / Soft-hydrothermal processing of red cedar bedding reduces its induction of cytochrome P450 in mouse liver. In: Laboratory Animals. 2009 ; Vol. 43, No. 2. pp. 205-211.
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