Soluble P-selectin is present in normal circulation and its plasma level is elevated in patients with thrombotic thrombocytopenic purpura and haemolytic uraemic syndrome

M. Katayama, M. Handa, Y. Araki, H. Ambo, Y. Kawai, K. Watanabe, Y. Ikeda

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Abstract

P-selectin is an integral membrane glycoprotein stored in the secretory granules of platelets and endothelial cells. To determine whether soluble P-selectin may be present in the circulation of healthy humans, we used a sandwich immunoassay to assess citrated plasma from 50 subjects. P-selectin was present in concentrations ranging from 19 to 521 ng/ml (mean ± SD = 121 ± 84 ng/ml). The apparent molecular weight of P-selectin immunoisolated from platelet poor plasma was similar to that of the detergent-soluble form isolated from platelet membrane. Plasma levels of P-selectin were unaffected by the following procedures: (1) drawing of blood in the presence of protease inhibitors; (2) stimulation of platelet-rich plasma with aggregating agents; (3) ultracentrifugation at 100000 g for 120 min at 4°C or filtration through a 0.22 μm membrane; or (4) preincubation of platelet-poor plasma with immobilized anti-platelet glycoprotein Ib monoclonal antibodies. It appeared that plasma P-selectin did not result from the in vitro activation of platelets, nor was it derived from platelet microparticles. We also found that plasma P-selectin levels were significantly elevated in patients with thrombotic thrombocytopenic purpura (12 patients, 332 ± 184 ng/ml, P < 0.001) and haemolytic uraemic syndrome (17 patients, 297 ± 191 ng/ml, P < 0.0001), as compared to the normal levels. Thus, these data should facilitate the study of the pathophysiological significance of circulating P-selectin.

Original languageEnglish
Pages (from-to)702-710
Number of pages9
JournalBritish Journal of Haematology
Volume84
Issue number4
Publication statusPublished - 1993

ASJC Scopus subject areas

  • Hematology

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