Solution exchange of droplet contacting lipid bilayer system

Yutaro Tsuji, Ryuji Kawano, Toshihisa Osaki, Hirotaka Sasaki, Koki Kamiya, Norihisa Miki, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This paper describes a bilayer lipid membranes (BLMs) chip capable of solution exchange. In the previous report, we successfully reconstituted α-hemolysin (αHL) in BLMs using droplets contacting method with an array device. For drug screening, solution exchange is required to test the effect of inhibitors or promoters for proteins. In this study, we attempted to exchange the solution in a droplet using a microfluidic channel (Figure 1). We could successfully inject desired solution into the droplet forming BLMs and to withdraw the initial solution out of a droplet. The electrical signal of αHL with its some blockers which was injected later instead of the initial signal was successfully recorded. We consider that this method will enable more accurate and efficient drug screening system using artificially BLMs and reconstituted membrane protein.

Original languageEnglish
Title of host publicationProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Pages882-885
Number of pages4
DOIs
Publication statusPublished - 2012
Event2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 - Paris, France
Duration: 2012 Jan 292012 Feb 2

Other

Other2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
CountryFrance
CityParis
Period12/1/2912/2/2

Fingerprint

Lipid bilayers
lipids
Ion exchange
Membrane Lipids
membranes
Hemolysin Proteins
Screening
drugs
screening
proteins
Proteins
Microfluidics
Pharmaceutical Preparations
inhibitors
Membrane Proteins
chips
Membranes

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Tsuji, Y., Kawano, R., Osaki, T., Sasaki, H., Kamiya, K., Miki, N., & Takeuchi, S. (2012). Solution exchange of droplet contacting lipid bilayer system. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) (pp. 882-885). [6170327] https://doi.org/10.1109/MEMSYS.2012.6170327

Solution exchange of droplet contacting lipid bilayer system. / Tsuji, Yutaro; Kawano, Ryuji; Osaki, Toshihisa; Sasaki, Hirotaka; Kamiya, Koki; Miki, Norihisa; Takeuchi, Shoji.

Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2012. p. 882-885 6170327.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tsuji, Y, Kawano, R, Osaki, T, Sasaki, H, Kamiya, K, Miki, N & Takeuchi, S 2012, Solution exchange of droplet contacting lipid bilayer system. in Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)., 6170327, pp. 882-885, 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012, Paris, France, 12/1/29. https://doi.org/10.1109/MEMSYS.2012.6170327
Tsuji Y, Kawano R, Osaki T, Sasaki H, Kamiya K, Miki N et al. Solution exchange of droplet contacting lipid bilayer system. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2012. p. 882-885. 6170327 https://doi.org/10.1109/MEMSYS.2012.6170327
Tsuji, Yutaro ; Kawano, Ryuji ; Osaki, Toshihisa ; Sasaki, Hirotaka ; Kamiya, Koki ; Miki, Norihisa ; Takeuchi, Shoji. / Solution exchange of droplet contacting lipid bilayer system. Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2012. pp. 882-885
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