Solution exchange of droplet contacting lipid bilayer system

Yutaro Tsuji, Ryuji Kawano, Toshihisa Osaki, Hirotaka Sasaki, Koki Kamiya, Norihisa Miki, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This paper describes a bilayer lipid membranes (BLMs) chip capable of solution exchange. In the previous report, we successfully reconstituted α-hemolysin (αHL) in BLMs using droplets contacting method with an array device. For drug screening, solution exchange is required to test the effect of inhibitors or promoters for proteins. In this study, we attempted to exchange the solution in a droplet using a microfluidic channel (Figure 1). We could successfully inject desired solution into the droplet forming BLMs and to withdraw the initial solution out of a droplet. The electrical signal of αHL with its some blockers which was injected later instead of the initial signal was successfully recorded. We consider that this method will enable more accurate and efficient drug screening system using artificially BLMs and reconstituted membrane protein.

Original languageEnglish
Title of host publication2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Pages882-885
Number of pages4
DOIs
Publication statusPublished - 2012 May 7
Event2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 - Paris, France
Duration: 2012 Jan 292012 Feb 2

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

Other2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
CountryFrance
CityParis
Period12/1/2912/2/2

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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  • Cite this

    Tsuji, Y., Kawano, R., Osaki, T., Sasaki, H., Kamiya, K., Miki, N., & Takeuchi, S. (2012). Solution exchange of droplet contacting lipid bilayer system. In 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 (pp. 882-885). [6170327] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2012.6170327