Solution structure of a GAAG tetraloop in helix 6 of SRP RNA from Pyrococcus furiosus

Kiyoshi Okada, Mari Takahashi, Taiichi Sakamoto, Gota Kawai, Kouji Nakamura, Akio Kanai

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The NMR structure of a 12-mer RNA derived from the helix 6 of SRP RNA from Pyrococcus furiosus , whose loop-closing base pair is U:G, was determined, and the structural and thermodynamic properties of the RNA were compared with those of a mutant RNA with the C:G closing base pair. Although the structures of the two RNAs are similar to each other and adopt the GNRR motif, the conformational stabilities are significantly different to each other. It was suggested that weaker stacking interaction of the GAAG loop with the U:G closing base pair in 12-mer RNA causes the lower conformational stability.

Original languageEnglish
Pages (from-to)383-395
Number of pages13
JournalNucleosides, Nucleotides and Nucleic Acids
Volume25
Issue number4-6
DOIs
Publication statusPublished - 2006 Jun 1

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Pyrococcus furiosus
RNA
Base Pairing
Thermodynamics
Structural properties
Thermodynamic properties
Nuclear magnetic resonance

Keywords

  • GNRR motif
  • SRP

ASJC Scopus subject areas

  • Genetics
  • Biochemistry

Cite this

Solution structure of a GAAG tetraloop in helix 6 of SRP RNA from Pyrococcus furiosus. / Okada, Kiyoshi; Takahashi, Mari; Sakamoto, Taiichi; Kawai, Gota; Nakamura, Kouji; Kanai, Akio.

In: Nucleosides, Nucleotides and Nucleic Acids, Vol. 25, No. 4-6, 01.06.2006, p. 383-395.

Research output: Contribution to journalArticle

Okada, Kiyoshi ; Takahashi, Mari ; Sakamoto, Taiichi ; Kawai, Gota ; Nakamura, Kouji ; Kanai, Akio. / Solution structure of a GAAG tetraloop in helix 6 of SRP RNA from Pyrococcus furiosus. In: Nucleosides, Nucleotides and Nucleic Acids. 2006 ; Vol. 25, No. 4-6. pp. 383-395.
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