Somatic Primary piRNA Biogenesis Driven by cis-Acting RNA Elements and trans-Acting Yb

Hirotsugu Ishizu, Yuka Iwasaki, Shigeki Hirakata, Haruka Ozaki, Wataru Iwasaki, Haruhiko Siomi, Mikiko C. Siomi

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Primary piRNAs in Drosophila ovarian somatic cells arise from piRNA cluster transcripts and the 3' UTRs of a subset of mRNAs, including Traffic jam (Tj) mRNA. However, it is unclear how these RNAs are determined as primary piRNA sources. Here, we identify a cis-acting 100-nt fragment in the Tj 3' UTR that is sufficient for producing artificial piRNAs from unintegrated DNA. These artificial piRNAs were effective in endogenous gene transcriptional silencing. Yb, a core component of primary piRNA biogenesis center Yb bodies, directly bound the Tj-cis element. Disruption of this interaction markedly reduced piRNA production. Thus, Yb is the trans-acting partner of the Tj-cis element. Yb-CLIP revealed that Yb binding correlated with somatic piRNA production but Tj-cis element downstream sequences produced few artificial piRNAs. We thus propose that Yb determines primary piRNA sources through two modes of action: primary binding to cis elements to specify substrates and secondary binding to downstream regions to increase diversity in piRNA populations.

Original languageEnglish
Pages (from-to)429-440
Number of pages12
JournalCell Reports
Volume12
Issue number3
DOIs
Publication statusPublished - 2015 Jul 21

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Small Interfering RNA
RNA
3' Untranslated Regions
Messenger RNA
Gene Silencing
Drosophila
Genes
DNA
Substrates
Population

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Somatic Primary piRNA Biogenesis Driven by cis-Acting RNA Elements and trans-Acting Yb. / Ishizu, Hirotsugu; Iwasaki, Yuka; Hirakata, Shigeki; Ozaki, Haruka; Iwasaki, Wataru; Siomi, Haruhiko; Siomi, Mikiko C.

In: Cell Reports, Vol. 12, No. 3, 21.07.2015, p. 429-440.

Research output: Contribution to journalArticle

Ishizu, Hirotsugu ; Iwasaki, Yuka ; Hirakata, Shigeki ; Ozaki, Haruka ; Iwasaki, Wataru ; Siomi, Haruhiko ; Siomi, Mikiko C. / Somatic Primary piRNA Biogenesis Driven by cis-Acting RNA Elements and trans-Acting Yb. In: Cell Reports. 2015 ; Vol. 12, No. 3. pp. 429-440.
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