Abstract
Lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. The major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. Apparent Km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 μM and 6.2 μM respectively. Apparent Vmax values were 14 pmol/min/mg protein for the cytosol enzyme and 32 pmol/min/mg protein for the microsomal enzyme. Net activities of cytosol and microsomal 12-lipoxygenase were 214 and 109 pmol/min/g wet tissue, respectively. Both cytosol and microsomal lipoxygenase activities were neither dependent on calcium nor ATP. Carbon monoxide failed to affect these enzyme activities. There were considerable differences either in the effect of glutathione or in the sensitivities toward several lipoxygenase inhibitors, indicating that the cytosol and the microsomal 12-lipoxygenase activities are derived from two different enzymes. Alternatively, the differences could be attributable to the different microenvironments of these enzymes.
| Original language | English |
|---|---|
| Pages (from-to) | 305-319 |
| Number of pages | 15 |
| Journal | Prostaglandins, Leukotrienes and Medicine |
| Volume | 21 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1986 |
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ASJC Scopus subject areas
- Biochemistry
- Endocrinology
- Physiology
Cite this
Some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate. / Nakadate, Teruo; Yokota, Eriko; Yamamoto, Satoshi; Kato, Ryuichi.
In: Prostaglandins, Leukotrienes and Medicine, Vol. 21, No. 3, 1986, p. 305-319.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate
AU - Nakadate, Teruo
AU - Yokota, Eriko
AU - Yamamoto, Satoshi
AU - Kato, Ryuichi
PY - 1986
Y1 - 1986
N2 - Lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. The major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. Apparent Km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 μM and 6.2 μM respectively. Apparent Vmax values were 14 pmol/min/mg protein for the cytosol enzyme and 32 pmol/min/mg protein for the microsomal enzyme. Net activities of cytosol and microsomal 12-lipoxygenase were 214 and 109 pmol/min/g wet tissue, respectively. Both cytosol and microsomal lipoxygenase activities were neither dependent on calcium nor ATP. Carbon monoxide failed to affect these enzyme activities. There were considerable differences either in the effect of glutathione or in the sensitivities toward several lipoxygenase inhibitors, indicating that the cytosol and the microsomal 12-lipoxygenase activities are derived from two different enzymes. Alternatively, the differences could be attributable to the different microenvironments of these enzymes.
AB - Lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. The major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. Apparent Km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 μM and 6.2 μM respectively. Apparent Vmax values were 14 pmol/min/mg protein for the cytosol enzyme and 32 pmol/min/mg protein for the microsomal enzyme. Net activities of cytosol and microsomal 12-lipoxygenase were 214 and 109 pmol/min/g wet tissue, respectively. Both cytosol and microsomal lipoxygenase activities were neither dependent on calcium nor ATP. Carbon monoxide failed to affect these enzyme activities. There were considerable differences either in the effect of glutathione or in the sensitivities toward several lipoxygenase inhibitors, indicating that the cytosol and the microsomal 12-lipoxygenase activities are derived from two different enzymes. Alternatively, the differences could be attributable to the different microenvironments of these enzymes.
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UR - http://www.scopus.com/inward/citedby.url?scp=0022570277&partnerID=8YFLogxK
U2 - 10.1016/0262-1746(86)90051-X
DO - 10.1016/0262-1746(86)90051-X
M3 - Article
C2 - 3085110
AN - SCOPUS:0022570277
VL - 21
SP - 305
EP - 319
JO - Prostaglandins Leukotrienes and Essential Fatty Acids
JF - Prostaglandins Leukotrienes and Essential Fatty Acids
SN - 0952-3278
IS - 3
ER -