Sox21 promotes hippocampal adult neurogenesis via the transcriptional repression of the Hes5 gene

Satoru Matsuda, Ken ichiro Kuwako, Hirotaka James Okano, Shuichi Tsutsumi, Hiroyuki Aburatani, Yumiko Saga, Yumi Matsuzaki, Akinori Akaike, Hachiro Sugimoto, Hideyuki Okano

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Despite the importance of the production of new neurons in the adult hippocampus, the transcription network governing this process remains poorly understood. The High Mobility Group (HMG)-box transcription factor, Sox2, and the cell surface activated transcriptional regulator, Notch, play important roles in CNS stem cells. Here, we demonstrate that another member of the SoxB (Sox1/Sox2/Sox3) transcription factor family, Sox21, is also a critical regulator of adult neurogenesis in mouse hippocampus. Loss of Sox21 impaired transition of progenitor cells from type 2a to type 2b, thereby reducing subsequent production of new neurons in the adult dentate gyrus. Analysis of the Sox21 binding sites in neural stem/progenitor cells indicated that the Notch-responsive gene, Hes5, was a target of Sox21. Sox21 repressed Hes5 gene expression at the transcriptional level. Simultaneous overexpression of Hes5 and Sox21 revealed that Hes5 was a downstream effector of Sox21 at the point where the Notch and Sox pathways intersect to control the number of neurons in the adult hippocampus. Therefore, Sox21 controls hippocampal adult neurogenesis via transcriptional repression of the Hes5 gene.

Original languageEnglish
Pages (from-to)12543-12557
Number of pages15
JournalJournal of Neuroscience
Volume32
Issue number36
DOIs
Publication statusPublished - 2012 Sep 5

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Neurogenesis
Hippocampus
Stem Cells
Neurons
Transcription Factors
Genes
Neural Stem Cells
Dentate Gyrus
Binding Sites
Gene Expression

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Sox21 promotes hippocampal adult neurogenesis via the transcriptional repression of the Hes5 gene. / Matsuda, Satoru; Kuwako, Ken ichiro; Okano, Hirotaka James; Tsutsumi, Shuichi; Aburatani, Hiroyuki; Saga, Yumiko; Matsuzaki, Yumi; Akaike, Akinori; Sugimoto, Hachiro; Okano, Hideyuki.

In: Journal of Neuroscience, Vol. 32, No. 36, 05.09.2012, p. 12543-12557.

Research output: Contribution to journalArticle

Matsuda, S, Kuwako, KI, Okano, HJ, Tsutsumi, S, Aburatani, H, Saga, Y, Matsuzaki, Y, Akaike, A, Sugimoto, H & Okano, H 2012, 'Sox21 promotes hippocampal adult neurogenesis via the transcriptional repression of the Hes5 gene', Journal of Neuroscience, vol. 32, no. 36, pp. 12543-12557. https://doi.org/10.1523/JNEUROSCI.5803-11.2012
Matsuda, Satoru ; Kuwako, Ken ichiro ; Okano, Hirotaka James ; Tsutsumi, Shuichi ; Aburatani, Hiroyuki ; Saga, Yumiko ; Matsuzaki, Yumi ; Akaike, Akinori ; Sugimoto, Hachiro ; Okano, Hideyuki. / Sox21 promotes hippocampal adult neurogenesis via the transcriptional repression of the Hes5 gene. In: Journal of Neuroscience. 2012 ; Vol. 32, No. 36. pp. 12543-12557.
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