TY - JOUR
T1 - Sp1-mediated transcription regulation of TAF-Iα gene encoding a histone chaperone
AU - Asaka, Masamitsu N.
AU - Murano, Kensaku
AU - Nagata, Kyosuke
N1 - Funding Information:
This work was supported in part by grant-in-aid from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (K.N.).
PY - 2008/11/28
Y1 - 2008/11/28
N2 - TAF-I, one of histone chaperones, consists of two subtypes, TAF-Iα and TAF-Iβ. The histone chaperone activity of TAF-I is regulated by dimer patterns of these subtypes. TAF-Iβ is expressed ubiquitously, while the expression level of TAF-Iα with less activity than TAF-Iβ differs among cell types. It is, therefore, assumed that the expression level of TAF-Iα in a cell is important for the TAF-I activity level. Here, we found that TAF-Iα and TAF-Iβ genes are under the control of distinct promoters. Reporter assays and gel shift assays demonstrated that Sp1 binds to three regions in the TAF-Iα promoter and two or all mutaions of the three Sp1 binding regions reduced the TAF-Iα promoter activity. ChIP assays demonstrated that Sp1 binds to the TAF-Iα promoter in vivo. Furthermore, the expression level of TAF-Iα mRNA was reduced by knockdown of Sp1 using siRNA method. These studies indicated that the TAF-Iα promoter is under the control of Sp1.
AB - TAF-I, one of histone chaperones, consists of two subtypes, TAF-Iα and TAF-Iβ. The histone chaperone activity of TAF-I is regulated by dimer patterns of these subtypes. TAF-Iβ is expressed ubiquitously, while the expression level of TAF-Iα with less activity than TAF-Iβ differs among cell types. It is, therefore, assumed that the expression level of TAF-Iα in a cell is important for the TAF-I activity level. Here, we found that TAF-Iα and TAF-Iβ genes are under the control of distinct promoters. Reporter assays and gel shift assays demonstrated that Sp1 binds to three regions in the TAF-Iα promoter and two or all mutaions of the three Sp1 binding regions reduced the TAF-Iα promoter activity. ChIP assays demonstrated that Sp1 binds to the TAF-Iα promoter in vivo. Furthermore, the expression level of TAF-Iα mRNA was reduced by knockdown of Sp1 using siRNA method. These studies indicated that the TAF-Iα promoter is under the control of Sp1.
KW - Adenovirus
KW - Chromatin remodeling
KW - SET
KW - TAF-I
KW - Transcription factor
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U2 - 10.1016/j.bbrc.2008.09.053
DO - 10.1016/j.bbrc.2008.09.053
M3 - Article
C2 - 18809386
AN - SCOPUS:53449089985
SN - 0006-291X
VL - 376
SP - 665
EP - 670
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -