TY - JOUR
T1 - Spatial and temporal control of laminin-332 and -511 expressions during hair morphogenesis
AU - Imanishi, Hisayoshi
AU - Tsuruta, Daisuke
AU - Tateishi, Chiharu
AU - Sugawara, Koji
AU - Kobayashi, Hiromi
AU - Ishii, Masamitsu
AU - Kishi, Kazuo
PY - 2014/3
Y1 - 2014/3
N2 - Hair is one of the smallest organs, but has many important functions to mammals. Hair morphogenesis occurs through the reciprocal exchange of epithelial and mesenchymal signals. There are some reports about the expression of laminin-511 and -332 during hair morphogenesis, but are no reports of the chronological expression and function of laminin-511 and its counter regulator laminin-332 during hair morphogenesis. Our results of immunoblotting revealed that laminin-332 proteins were detected at stage 0 and downregulated during stage 1 to stage 2, and then recovered at stage 3. However, laminin α5 expression was constant throughout stages 0-3. According to the results of semi-quantitative RT-PCR, the mRNA expression of all laminin-332 subunits increased gradually from stage 0 to stage 2, while the mRNA expression of all laminin-511 subunits remained constant from stage 0 to stage 3. Our results suggest that the proper expression of laminin-332 and laminin-511 may regulate appropriate hair morphogenesis.
AB - Hair is one of the smallest organs, but has many important functions to mammals. Hair morphogenesis occurs through the reciprocal exchange of epithelial and mesenchymal signals. There are some reports about the expression of laminin-511 and -332 during hair morphogenesis, but are no reports of the chronological expression and function of laminin-511 and its counter regulator laminin-332 during hair morphogenesis. Our results of immunoblotting revealed that laminin-332 proteins were detected at stage 0 and downregulated during stage 1 to stage 2, and then recovered at stage 3. However, laminin α5 expression was constant throughout stages 0-3. According to the results of semi-quantitative RT-PCR, the mRNA expression of all laminin-332 subunits increased gradually from stage 0 to stage 2, while the mRNA expression of all laminin-511 subunits remained constant from stage 0 to stage 3. Our results suggest that the proper expression of laminin-332 and laminin-511 may regulate appropriate hair morphogenesis.
KW - Extracellular matrix
KW - Hair morphogenesis
KW - Laminin-332
KW - Laminin-511
KW - α3 integrin
KW - β4 integrin
UR - http://www.scopus.com/inward/record.url?scp=84897028514&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84897028514&partnerID=8YFLogxK
U2 - 10.1007/s00795-013-0040-1
DO - 10.1007/s00795-013-0040-1
M3 - Article
C2 - 23529140
AN - SCOPUS:84897028514
SN - 1860-1480
VL - 47
SP - 38
EP - 42
JO - Medical Molecular Morphology
JF - Medical Molecular Morphology
IS - 1
ER -